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Published on June 26, 2008; 10.1104/pp.108.123273

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Received May 22, 2008
Accepted June 10, 2008

Silencing onion lachrymatory factor synthase causes a significant change in the sulfur secondary metabolite profile

Colin C. Eady *, Takahiro Kamoi , Masahiro Kato , Noel G. Porter , Sheree Davis , Martin Shaw , Akiko Kamoi , and Shinsuke Imai

National Centre for Advanced Bio-Protection Technologies, Lincoln University, Christchurch, New Zealand; New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand; House Foods Corporation, 1-4 Takanodai, Yotsukaido, Chiba, Japan 284-0033

* Corresponding author; email: eadyc{at}crop.cri.nz.

Through a single genetic transformation in onion, a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene (lfs) using RNAi silencing in six plants. This reduced lachrymatory synthase activity by up to 1544-fold, so that when wounded the onions produced significantly reduced levels of tear-inducing lachrymatory factor. We then confirmed, through a novel colorimetric assay, that this silencing had shifted the trans-S-1-propenyl-L-cysteine sulfoxide (1-PRENCSO) breakdown pathway so that more 1-propenyl sulfenic acid was converted into di-1-propenyl thiosulfinate. A consequence of this raised thiosulfinate level was a marked increase in the downstream production of a non-enzymatically produced zwiebelane isomer and other volatile sulfur compounds, di-1-propenyl disulfide and 2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, which had previously been reported either in trace amounts or had not been detected in onion. The consequences of this dramatic simultaneous down- and up-regulation of secondary sulfur products on the health and flavour attributes of the onion are discussed.




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R. A. Musah, Q. He, and R. Kubec
Discovery and Characterization of a Novel Lachrymatory Factor Synthase in Petiveria alliacea and Its Influence on Alliinase-Mediated Formation of Biologically Active Organosulfur Compounds
Plant Physiology, November 1, 2009; 151(3): 1294 - 1303.
[Abstract] [Full Text] [PDF]


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