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Published on August 1, 2008; 10.1104/pp.108.123836

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Received June 1, 2008
Accepted July 27, 2008

Involvement of the Pepper Antimicrobial Protein CaAMP1 Gene in Broad Spectrum Disease Resistance

Sung Chul Lee , In Sun Hwang , Hyong Woo Choi , and Byung Kook Hwang *

Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea

* Corresponding author; email: bkhwang{at}korea.ac.kr.

Pathogen-inducible antimicrobial defense-related proteins have emerged as key antibiotic peptides and enzymes involved in disease resistance in plants. A novel antimicrobial protein gene, CaAMP1, was isolated from pepper (Capsicum annuum) leaves infected with Xanthomonas campestris pv. vesicatoria (Xcv). Expression of the CaAMP1 gene was strongly induced in pepper leaves not only during pathogen infection but also after exposure to abiotic elicitors. The purified recombinant CaAMP1 protein possessed broad spectrum antimicrobial activity against phytopathogenic bacteria and fungi. CaAMP1:smGFP fusion protein was mainly localized in the external and intercellular regions of the onion epidermal cells. The virus-induced gene silencing technique and gain-of-function transgenic plants were used to determine the CaAMP1 gene function in plant defense. Silencing of CaAMP1 led to enhanced susceptibility to Xcv and Colletotrichum coccodes infection, accompanied by reduced PR gene expression. In contrast, overexpression of CaAMP1 in Arabidopsis thaliana conferred broad spectrum resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst), the biotrophic oomycete Hyaloperonospora parasitica, and the fungal necrotrophic pathogens Fusarium oxysporum f.sp. matthiolae and Alternaria brassicicola. CaAMP1 overexpression induced the SA pathway-dependent genes PR1 and PR5, but not the jasmonic acid-dependent defense gene PDF1.2 during Pst infection. Together, these results suggest that the antimicrobial CaAMP1 protein is involved in broad spectrum resistance to bacterial and fungal pathogen infection.




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