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Published on September 17, 2008; 10.1104/pp.108.124420

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Received June 11, 2008
Accepted September 10, 2008

The Pollen Receptor Kinase LePRK2 Mediates Growth-Promoting Signals and Positively Regulates Pollen Germination and Tube Growth

Dong Zhang , Diego Wengier , Bin Shuai , Cai-Ping Gui , Jorge Muschietti , Sheila McCormick , and Wei-Hua Tang *

SIBS-UC (Berkeley) Center of Molecular Life Sciences, National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, P.R. China; Instituto de Ingenieria Genetica y Biologia Molecular (INGEBI), CONICET Departamento de Fisiologia y Biologia Molecular y Celular FCEN, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina; Plant Gene Expression Center, United States Department of Agriculture/Agricultural Research Service, and Department of Plant and Microbial Biology, University of California at Berkeley, 800 Buchanan Street, Albany, California 94710

* Corresponding author; email: whtang{at}sibs.ac.cn.

In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato, LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes can not be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to STIL, an extracellular growth-promoting signal from the pistil. Pollen tubes transiently over-expressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes co-expressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals.




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Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2008
J. Exp. Bot., June 23, 2009; (2009) erp154v1.
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