Plant Physiology Preview
Published on July 16, 2008; 10.1104/pp.108.124545
OPEN ACCESS ARTICLE
Received June 11, 2008
Accepted July 4, 2008
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis thaliana
Heidi Rutschow , A. Jimmy Ytterberg , Giulia Friso , Robert Nilsson , and Klaas J. van Wijk *
Department of Plant Biology, Cornell University, Ithaca, New York 14853
* Corresponding author; email: kv35{at}cornell.edu.
cpSRP54 is involved in co- and post-translational sorting of thylakoid proteins. The Arabidopsis cpSRP54 null mutant, ffc1-2, is pale green with delayed development. Western blot analysis of individual leaves showed that the SRP sorting pathway, but not the SecYE translocon, was strongly down-regulated with progressive leaf development in both wild-type and ffc1-2. To further understand the impact of cpSRP54 deletion, a quantitative comparison between ffc2-1 was carried out for total leaf proteomes of young seedlings and for chloroplast proteomes of fully developed leaves, using stable isotope labeling (iTRAQ and ICAT) and 2-dimensional gels. This showed that cpSRP54 deletion led to a change in LHC composition, increase of PsbS, and a decreased Photosystem I/II ratio. Moreover, the cpSRP54 deletion led in young leaves to up-regulation of thylakoid proteases and stromal chaperones, including ClpC. In contrast, the stromal protein homeostasis machinery returned to wild-type levels in mature leaves, consistent with the developmental down-regulation of the SRP pathway. A differential response between young and mature leaves was also found in carbon metabolism, with an up-regulation of the Calvin cycle and the photorespiratory pathway in peroxisomes and mitochondria in young leaves but not in old leaves. In contrast, the Calvin cycle was down-regulated in mature leaves, likely to adjust to the reduced capacity of the light reaction, while reactive oxygen species defense proteins were up-regulated. The significance of ClpC up-regulation was confirmed through the generation of an ffc2-1 x clpc1 double mutant. This mutant was seedling-lethal under autotrophic conditions, but could be partially rescued under heterotrophic conditions.
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