Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 13, 2008; 10.1104/pp.108.125294

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
148/2/843    most recent
pp.108.125294v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, Y.
Right arrow Articles by Chong, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, Y.
Right arrow Articles by Chong, K.
Agricola
Right arrow Articles by Han, Y.
Right arrow Articles by Chong, K.

Received June 24, 2008
Accepted August 9, 2008

The Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-box and Proteasome Dependent Manner

Ye Han , Hong Cao , Jiafu Jiang , Yunyuan Xu , Jizhou Du , Xin Wang , Ming Yuan , Zhiyong Wang , Zhihong Xu , and Kang Chong *

Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China; Department Plant Biology, Carnegie Institution of Washington and Department of Biological Sciences, Stanford University, Stanford CA 94305, USA; National Research Center for Plant Gene, Beijing 100093, China; and Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

* Corresponding author; email: chongk{at}ibcas.ac.cn.

Root growth is mainly determined by cell division and subsequent elongation in the root apical area. Components regulating cell division in the root meristematic cells are largely unknown. Previous studies have identified OsRAA1 as a regulator in root development. Yet the function of OsRAA1 at the cellular and molecular level is unclear. Here, we show that OsRAA1 overexpressed transgenic rice showed reduced primary root growth, increased number of cells in metaphase, and reduced number of cells in anaphase, which suggests that OsRAA1 is responsible for limiting root growth by inhibiting the onset of anaphase. The expression of OsRAA1 in fission yeast also induced metaphase arrest, which is consistent with the fact that OsRAA1 functions through a conserved mechanism of cell cycle regulation. Moreover, co-localization assay has shown that OsRAA1 expresses predominantly at spindles during cell division. Yeast two-hybrid and pull-down, as well as bimolecular fluorescent complementation assays all have revealed that OsRAA1 interacts with a rice homolog of RPT4, a component that is involved in ubiquitin pathway. Treating transgenic rice with specific inhibitors of 26S proteasome blocked the degradation of OsRAA1 and increased the number of cells in metaphase. Mutation of a putative ubiquitination-targeting D-box (RGSLDLISL) in OsRAA1 interrupted the destruction of OsRAA1 in transgenic yeast. The results suggest that ubiquitination and proteasomic proteolysis are involved in the OsRAA1 degradation which is essential for the onset of anaphase and that OsRAA1 may modulate root development mediated by the ubiquitin-proteasome pathway as a novel regulatory factor of the cell cycle.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists