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Published on August 8, 2008; 10.1104/pp.108.125567

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Received June 30, 2008
Accepted August 1, 2008

Heme oxygenase/carbon monoxide system is involved in auxin-induced cucumber adventitious rooting process

Wei Xuan , Fu-Yuan Zhu , Sheng Xu , Ben-Kai Huang , Teng-Fang Ling , Ji-Yan Qi , Mao-Bing Ye , and Wen-Biao Shen *

College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China

* Corresponding author; email: wbshenh{at}njau.edu.cn.

IAA is an important regulator of the adventitious rooting via activation of complex signaling cascades. In animals, carbon monoxide (CO), mainly generated by heme oxygenases (HOs), is a significant modulator of inflammatory reactions, affecting cell proliferation and production of growth factors. In this report, we showed that the treatment with the auxin transport inhibitor naphthylphthalamic acid (NPA) besides prevented auxin-mediated induction of adventitious rooting, also decreased the activity of HO and its by-product CO content. The application of IAA, HO-1 activator/CO donor hematin or CO aqueous solution was able to alleviate the IAA depletion-induced inhibition of adventitious root formation. Meanwhile, IAA or hematin treatment rapidly activated HO activity or HO-1 protein expression, and CO content was also enhanced. The application of HO-1 specific inhibitor ZnPPIX could inhibit above IAA and hematin responses. While, CO aqueous solution treatment was able to ameliorate ZnPPIX-induced the inhibition of adventitious rooting. Molecular evidence further showed that ZnPPIX mimicked the effects of NPA on the inhibition of adventitious rooting, the down-regulation of one DnaJ-like gene CSDNAJ-1 and two calcium dependent protein kinases genes CSCDPK1/5. Application of CO aqueous solution could not only dose-dependently block IAA depletion-induced inhibition of adventitious rooting, but also enhance endogenous CO content and up-regulate CSDNAJ-1 and CSCDPK1/5 transcripts. Together, we provided pharmacological, physiological and molecular evidence that auxin rapidly activates HO activity and that the product of HO action, CO then triggers the signal transduction events that lead to the auxin responses of adventitious root formation in cucumber.






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