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Published on August 27, 2008; 10.1104/pp.108.125633

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Received July 1, 2008
Accepted August 25, 2008

Dynamic Proteomic Analysis Reveals a Switch Between Central Carbon Metabolism and Alcoholic Fermentation in Oryza sativa Filling Grains

Sheng Bao Xu , Tang Li , Zhu Yun Deng , Kang Chong , Yongbiao Xue , and Tai Wang *

Research Center of Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Beijing 10010, China; National Center for Plant Gene Research, Beijing 100093, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, China

* Corresponding author; email: twang{at}ibcas.ac.cn.

Accumulation of reserve materials in filling grains involves coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice is an excellent model for studying grain filling because of its importance as a staple food and the available genome sequence database. Our observations showed that embryo differentiation and endosperm cellularization in developing rice seeds were completed approximately 6 days after flowering (DAF); and thereafter, the immature seeds mainly underwent cell enlargement and reached the size of mature seeds at 12 DAF. Grain filling began at 6 DAF and lasted until 20 DAF. Dynamic proteomic analyses revealed 396 protein spots differentially expressed throughout 8 sequential developmental stages from 6 to 20 DAF, and determined 345 identities. These proteins were involved in different cellular and metabolic processes with a prominently functional skew toward metabolism (45%) and protein synthesis/destination (20%). Expression analyses of protein groups associated with different functional categories/subcategories showed that substantially up-regulated proteins were involved in starch synthesis and alcoholic fermentation; whereas the proteins down-regulated in the process involved in central carbon metabolism and most of the other functional categories/subcategories such as cell growth/division, protein synthesis, proteolysis and signal transduction. The coordinated changes were consistent with the transition from cell growth and differentiation to starch synthesis and clearly indicated that a switch from central carbon metabolism to alcoholic fermentation may be important for starch synthesis and accumulation in the developmental process.




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[Abstract] [Full Text] [PDF]


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