Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 17, 2008; 10.1104/pp.108.126003

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
148/3/1630    most recent
pp.108.126003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmidlin, L.
Right arrow Articles by Hugueney, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmidlin, L.
Right arrow Articles by Hugueney, P.
Agricola
Right arrow Articles by Schmidlin, L.
Right arrow Articles by Hugueney, P.

Received July 14, 2008
Accepted September 11, 2008

A Stress-Inducible Resveratrol O-Methyltransferases Involved in the Biosynthesis of Pterostilbene in Grapevine

Laure Schmidlin , Anne Poutaraud , Patricia Claudel , Pere Mestre , Emilce Prado , Maria Santos-Rosa , Sabine Wiedemann-Merdinoglu , Francis Karst , Didier Merdinoglu , and Philippe Hugueney *

Laboratoire de Genetique et d'Amelioration de la Vigne, UMR1131, INRA, Universite Louis Pasteur Strasbourg, 28 rue de Herrlisheim, F-68021 Colmar, France; Laboratoire d'Oenologie, UMR1131, INRA, Universite Louis Pasteur Strasbourg, 28 rue de Herrlisheim, F-68021 Colmar, France; Laboratoire Reproduction et Developpement des Plantes, IFR128, UMR5667, CNRS, INRA, Universite Lyon 1, Ecole Normale Superieure de Lyon, 46 allee d'Italie, 69364 Lyon Cedex 07, France

* Corresponding author; email: Philippe.Hugueney{at}ens-lyon.fr.

Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention, due to both its antifungal and pharmacological properties. Indeed, pterostilbene is 5 to 10 times more fungitoxic than resveratrol in vitro and recent studies have shown that pterostilbene exhibits anti-cancer, hypolipidemic and anti-diabetic properties. A candidate gene approach was used to identify a grapevine resveratrol O-methyltransferase (ROMT) cDNA and the activity of the corresponding protein was characterized, after expression in Escherichia coli. Transient co-expression of ROMT and grapevine stilbene synthase in tobacco using the agroinfiltration technique resulted in the accumulation of pterostilbene in tobacco tissues. Taken together these results showed that ROMT was able to catalyse the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. ROMT gene expression in grapevine leaves was induced by different stresses, including downy mildew (Plasmopara viticola) infection, UV light and AlCl3 treatment.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
P. Hugueney, S. Provenzano, C. Verries, A. Ferrandino, E. Meudec, G. Batelli, D. Merdinoglu, V. Cheynier, A. Schubert, and A. Ageorges
A Novel Cation-Dependent O-Methyltransferase Involved in Anthocyanin Methylation in Grapevine
Plant Physiology, August 1, 2009; 150(4): 2057 - 2070.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists