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Published on December 17, 2008; 10.1104/pp.108.126136

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Received July 30, 2008
Accepted December 4, 2008

In vivo interference with AtTCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development

Christine Herve , Patrick Dabos , Claude Bardet , Alain Jauneau , Marie Christine Auriac , Agnes Ramboer , Fabrice Lacout , and Dominique Tremousaygue *

IFR40 CNRS, Pole de Biotechnologie Vegetale, 24 chemin de Borde Rouge, BP 52627 31326 Castanet Tolosan Cedex; CNRS 2594, Laboratoire des Interactions Plantes Microorganismes (LIPM), INRA 441, 24 chemin de Borde Rouge, BP 52627 31326 Castanet Tolosan Cedex

* Corresponding author; email: dominique.tremousaygue{at}toulouse.inra.fr.

AtTCP20 is a transcription factor belonging to the TCP-P subfamily, characterized by its capacity to bind to site II motifs (TGGGCY). Our aim was to understand the role of AtTCP20 in plant development. The expression pattern of a translational fusion of PromTCP20:CDS20::GUS::GFP suggested a function for AtTCP20 in several plant organs and stages of development. The role of AtTCP20 was challenged in planta by inducing expression of AtTCP20 proteins fused with either a transcriptional activator domain (VP16) or a repressor domain (EAR). Expression of both modified proteins led to severe developmental phenotypes. In depth analysis suggested that AtTCP20 may participate in the regulation of cell expansion, cell division and cell differentiation. Gene expression profiling in roots and hypocotyls revealed that 252 genes were down-regulated in both organs after induction of the AtTCP20::EAR repressor gene. Site II motifs (TGGGCY) were under represented in their promoters. Conversely GG(A/T)CCC sequences related to binding sites identified for TCP proteins in rice were over represented and a TCP20 fusion protein was shown to bind to these sequences in vitro. Gene ontology indicated that many targeted genes were involved in cell wall biogenesis and modification during expansion and also encoded numerous transcription factors controlling plant development. Our results are consistent with the previous proposal that AtTCP20 is involved in cell division and growth coordination. Moreover they further suggest that AtTCP20 also contributes to cell expansion control and indicate a different involvement of this protein in plant morphogenesis depending on the organ and the developmental stage.






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