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Published on August 6, 2008; 10.1104/pp.108.126201

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Received July 10, 2008
Accepted August 4, 2008

Mitochondrial mRNA polymorphisms in different Arabidopsis thaliana accessions

Joachim Forner , Angela Holzle , Christian Jonietz , Sabine Thuss , Markus Schwarzlander , Barbel Weber , Rhonda C. Meyer , and Stefan Binder *

Institut Molekulare Botanik, Universitat Ulm, D-89069 Ulm, Germany; Molekulare Genetik, Leibniz-Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3 D-06466 Gatersleben Germany

* Corresponding author; email: stefan.binder{at}uni-ulm.de.

In our analysis of 5' and 3' end formation in plant mitochondria we compared the major transcript ends of all mitochondrial protein-coding genes between the three Arabidopsis thaliana accessions Columbia (Col), C24 and Landsberg erecta (Ler). Differences between transcript patterns were found for seven genes. For atp6-2 no transcripts at all were detected in Ler. This and further analyses suggest the atp6-2 gene arrangement to be absent from the mitochondrial DNA of this accession. All other transcript polymorphisms are attributed to variations at the 5' termini and were consistently observed in all tissues investigated. mRNA phenotyping of reciprocal Col/Ler, Col/C24 and Ler/C24 F1 hybrids revealed the differing transcript pattern of ccmC to be inherited maternally suggesting these to arise from differences in the mitochondrial DNA. Bi-parental inheritance was observed for the polymorphic transcripts of nad4, nad9, ccmB and rpl5 indicating these differences to be caused by nuclear encoded trans-factors. Deviant transcript patterns were tested in further accessions and were found in at least three additional accessions. Detailed examination of the nad4 and the nad9 transcripts demonstrates that the respective polymorphisms affect the major mRNAs of these genes. This study shows that natural genetic variation in Arabidopsis thaliana can also affect mitochondrial mRNA end processing. These variations can now be used to identify the nuclear genes responsible as well as the mitochondrial cis-elements required for 5' end generation of mitochondrial transcripts.




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