Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 7, 2008; 10.1104/pp.108.126276

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
149/1/499    most recent
pp.108.126276v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beßer, K.
Right arrow Articles by Broun, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beßer, K.
Right arrow Articles by Broun, P.
Agricola
Right arrow Articles by Beßer, K.
Right arrow Articles by Broun, P.

Received July 13, 2008
Accepted November 4, 2008

Divergent regulation of terpenoid metabolism in the trichomes of wild and cultivated tomato species

Katrin Beßer , Andrea Harper , Nicholas Welsby , Ines Schauvinhold , Stephen Slocombe , Yi Li , Richard A. Dixon , and Pierre Broun *

CNAP, Department of Biology, University of York, York YO10 5YW, UK; Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401 USA

* Corresponding author; email: pierre.broun{at}rdto.nestle.com.

The diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in tomato. We have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence in trichome metabolism between the wild species Solanum habrochaites LA1777 and its cultivated relative, S. lycopersicum. LA1777 produces high amounts of insecticidal sesquiterpene carboxylic acids (SCAs), whereas cultivated tomatoes lack SCAs and are more susceptible to pests. We show that trichomes of the two species have nearly opposite terpenoid profiles consisting of mainly monoterpenes and low levels of sesquiterpenes in S. lycopersicum and mainly SCAs and very low monoterpene levels in LA1777. The accumulation patterns of these terpenoids are different during development, in contrast to the developmental expression profiles of terpenoid pathway genes which are similar in the two species, but do not correlate in either case with terpenoid accumulation. However, our data suggest that the accumulation of monoterpenes in S. lycopersicum and major sesquiterpenes in LA1777 are linked both genetically and biochemically. Metabolite analyses after targeted gene silencing, inhibitor treatments, and precursor feeding all show that sesquiterpene biosynthesis relies mainly on products from the plastidic 2-C-methyl-D-erythritol-4-phosphate pathway in LA1777, but less so in the cultivated species. Furthermore, two classes of sesquiterpenes produced by the wild species may be synthesized from distinct pools of precursors via cytosolic and plastidial cyclases. However, highly trichome-expressed sesquiterpene cyclase-like enzymes were ruled out to be involved in the production of major LA1777 sesquiterpenes.




This article has been cited by other articles:


Home page
 J Exp BotHome page
M. L. Campos, M. de Almeida, M. L. Rossi, A. P. Martinelli, C. G. Litholdo Junior, A. Figueira, F. T. Rampelotti-Ferreira, J. D. Vendramim, V. A. Benedito, and L. E. Pereira Peres
Brassinosteroids interact negatively with jasmonates in the formation of anti-herbivory traits in tomato
J. Exp. Bot., November 1, 2009; 60(15): 4347 - 4361.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Breuer, A. Kawamura, T. Ichikawa, R. Tominaga-Wada, T. Wada, Y. Kondou, S. Muto, M. Matsui, and K. Sugimoto
The Trihelix Transcription Factor GTL1 Regulates Ploidy-Dependent Cell Growth in the Arabidopsis Trichome
PLANT CELL, August 1, 2009; 21(8): 2307 - 2322.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists