Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 6, 2008; 10.1104/pp.108.126789

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
148/2/719    most recent
pp.108.126789v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fahnenstich, H.
Right arrow Articles by Maurino, V. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fahnenstich, H.
Right arrow Articles by Maurino, V. G.
Agricola
Right arrow Articles by Fahnenstich, H.
Right arrow Articles by Maurino, V. G.

Received July 22, 2008
Accepted August 4, 2008

Generation of H2O2 in chloroplasts of Arabidopsis thaliana overexpressing glycolate oxidase as an inducible system to study oxidative stress

Holger Fahnenstich , Telma E. Scarpeci , Estela M. Valle , Ulf-Ingo Flugge , and Veronica G. Maurino *

Botanisches Institut, Universitat zu Koln, Gyrhofstr. 15, 50931, Cologne, Germany; Instituto de Biologia Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina

* Corresponding author; email: v.maurino{at}uni-koeln.de.

Arabidopsis thaliana overexpressing glycolate oxidase (GO) in chloroplasts accumulates both hydrogen peroxide (H2O2) and glyoxylate. GO overexpressing lines (GO plants) grown at 75 µmol quanta m-2 s-1 show retarded development, yellowish rosettes and impaired photosynthetic performance, while at 30 µmol quanta m-2 s-1 this phenotype virtually disappears. The GO plants develop oxidative stress lesions under photorespiratory conditions but grow like the wild-type under non-photorespiratory conditions. GO plants co-expressing enzymes which further metabolize glyoxylate but still accumulate H2O2 show all features of the GO phenotype indicating that H2O2 is responsible for the GO phenotype. The GO plants can complete their life cycle indicating that they are able to adapt to the stress conditions imposed by the accumulation of H2O2 during the light period. Moreover, the data indicate that a response to oxidative stress is installed, with increased expression and/or activity of known oxidative stress responsive components. Hence, the GO plants are an ideal non-invasive model system to study the effects of H2O2 directly in the chloroplasts because H2O2 accumulation is inducible and sustained perturbations can reproducibly be provoked by exposing the plants to different ambient conditions.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Engqvist, M. F. Drincovich, U.-I. Flugge, and V. G. Maurino
Two D-2-Hydroxy-acid Dehydrogenases in Arabidopsis thaliana with Catalytic Capacities to Participate in the Last Reactions of the Methylglyoxal and {beta}-Oxidation Pathways
J. Biol. Chem., September 11, 2009; 284(37): 25026 - 25037.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists