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Published on October 22, 2008; 10.1104/pp.108.126813

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Received July 22, 2008
Accepted October 16, 2008

Transcriptional modulation of ERF protein JERF3 in the oxidative stress response enhances tolerance of tobacco seedlings to salt, drought and freezing

Lijun Wu , Zhijin Zhang , Haiwen Zhang , Xue-Chen Wang , and Rongfeng Huang *

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China; National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China; National Center for Plant Gene Research (Beijing), Beijing 100081, China

* Corresponding author; email: rongfeng{at}public3.bta.net.cn.

Abiotic stresses such as drought, cold, and salinity affect normal growth and development in plants. The production and accumulation of reactive oxygen species (ROS) cause oxidative stress under these abiotic conditions. Recent research has elucidated the significant role of ethylene response factor (ERF) proteins in plant adaptation to abiotic stresses. Our earlier functional analysis of an ERF protein, JERF3, indicated that JERF3-expressing tobacco adapts better to salinity in vitro. This paper extends that study by showing that transcriptional regulation of JERF3 in the oxidative stress response modulates the increased tolerance to abiotic stresses. Firstly, we confirm that JERF3-expressing tobacco enhances adaptation to drought, freezing, and osmotic stress during germination and seedling development. Then we demonstrate that JERF3-expressing tobacco imparts not only higher expression of osmotic-stress genes compared to wild type tobacco, but also the activation of photosynthetic carbon assimilation/metabolism and oxidative genes. More importantly, this regulation of the expression of oxidative genes subsequently enhances the activities of SOD but reduces the content of ROS in tobacco under drought, cold, salt, and ABA treatments. This indicates that JERF3 also modulates the abiotic stress response via the regulation of the oxidative stress response. Further assays indicate that JERF3 activates the expression of reporter genes driven by the osmotic-responsive GCC box, DRE, CE1, and by oxidative responsive as-1 in transient assays, suggesting the transcriptional activation of JERF3 in the expression of genes involved in response to oxidative and osmotic stress. Our results therefore establish that JERF3 activates the expression of such genes through transcription, resulting in decreased accumulation of ROS and, in turn, enhanced adaptation to drought, freezing, and salt in tobacco.




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