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Published on December 24, 2008; 10.1104/pp.108.127183

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Received July 30, 2008
Accepted December 18, 2008

CYP86A33 targeted gene silencing in potato tuber alters suberin composition, distorts suberin lamellae and impairs the periderm's water barrier function

Olga Serra , Marcal Soler , Carolin Hohn , Vincent Sauveplane , Franck Pinot , Rochus Franke , Lukas Schreiber , Salome Prat , Marisa Molinas , and Merce Figueras *

Laboratori del Suro, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus Montilivi s/n, E-17071 Girona, Spain; Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany; CNRS-Universite Louis Pasteur, Institut de Biologie Moleculaire des Plantes, rue Goethe 28, F-67083 Strasbourg, France; Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus Universidad Autonoma de Madrid, c/ Darwin 3, E-28049 Madrid, Spain

* Corresponding author; email: merce.figueras{at}udg.edu.

Suberin is a cell wall lipid-polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was downregulated in potato plants by RNA interference (RNAi)-mediated silencing. Periderm from CYP86A33 silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 {omega}-hydroxyacid (~70%) and {alpha},{omega}-diacid (~90%) monomers in comparison with wild-type. Moreover the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33 silenced lines was 3.5 times higher than that of the wild-type. Thus, our data provide convincing evidence for the involvement of {omega}-functional fatty acids in establishing suberin structure and function.




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