Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 10, 2008; 10.1104/pp.108.127977

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
148/3/1523    most recent
pp.108.127977v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kocal, N.
Right arrow Articles by Sonnewald, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kocal, N.
Right arrow Articles by Sonnewald, S.
Agricola
Right arrow Articles by Kocal, N.
Right arrow Articles by Sonnewald, S.

Received August 11, 2008
Accepted September 5, 2008

Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv. vesicatoria

Nurcan Kocal , Uwe Sonnewald , and Sophia Sonnewald *

Friedrich-Alexander Universitat Erlangen-Nurnberg, Lehrstuhl fur Biochemie, Staudtstr. 5, 91058 Erlangen, Germany

* Corresponding author; email: ssonne{at}biologie.uni-erlangen.de.

Cell wall-bound invertase (cw-Inv) plays an important role in carbohydrate partitioning and regulation of sink-source interaction. There is increasing evidence that pathogens interfere with sink-source interaction and induction of cw-Inv activity has frequently been shown in response to pathogen infection. To investigate the role of cw-Inv, transgenic tomato plants silenced for the major leaf cw-Inv isoforms were generated and analysed during normal growth and during the compatible interaction with Xanthomonas campestris pv. vesicatoria (Xcv).

Under normal growth conditions, activities of sucrolytic enzymes as well as photosynthesis and respiration were unaltered in the transgenic plants, as compared to wild type. However, starch levels of source leaves were strongly reduced, which was most likely caused by an enhanced sucrose exudation rate.

Following Xcv infection cw-Inv silenced plants showed an increased sucrose-to-hexose ratio in the apoplast of leaves. Symptom development, inhibition of photosynthesis and expression of photosynthetic genes were clearly delayed in transgenic plants as compared to wild type. In addition, induction of senescence-associated and PR genes observed in infected wild type plants was abolished in cw-Inv silenced tomato lines. These changes were not associated with decreased bacterial growth.

In conclusion, cw-Inv restricts carbon export from source leaves and regulates the sucrose-to-hexose ratio in the apoplast. Furthermore, an increased apoplastic hexose-to-sucrose ratio can be linked to inhibition of photosynthesis and induction of PR gene expression but does not significantly influence bacterial growth. Indirectly, bacteria may benefit from low invertase activity since longevity of host cells is raised and basal defence might be dampened.




This article has been cited by other articles:


Home page
 Plant CellHome page
J.-G. Kim, X. Li, J. A. Roden, K. W. Taylor, C. D. Aakre, B. Su, S. Lalonde, A. Kirik, Y. Chen, G. Baranage, et al.
Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1
PLANT CELL, April 1, 2009; 21(4): 1305 - 1323.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Depuydt, S. Trenkamp, A. R. Fernie, S. Elftieh, J.-P. Renou, M. Vuylsteke, M. Holsters, and D. Vereecke
An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians
Plant Physiology, March 1, 2009; 149(3): 1366 - 1386.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists