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Published on November 5, 2008; 10.1104/pp.108.129635

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Received September 9, 2008
Accepted October 30, 2008

Flux balance analysis of barley seeds: a computational approach to study systemic properties of central metabolism

Eva Grafahrend-Belau *, Falk Schreiber , Dirk Koschutzki , and Bjorn H. Junker

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany; Martin-Luther-University Halle-Wittenberg, Universitatsplatz 11, 06099 Halle, Germany; Furtwangen University of Applied Sciences, Robert-Gerwig-Platz 1, 78120 Furtwangen, Germany

* Corresponding author; email: grafahr{at}ipk-gatersleben.de.

The accumulation of storage compounds is an important aspect of cereal seed metabolism. Due to the agronomical importance of the storage reserves of starch, protein and oil, the understanding of storage metabolism is of scientific interest with practical applications in agronomy and plant breeding. To get insight into storage patterning in developing cereal seed in response to environmental and genetic perturbation, a computational analysis of seed metabolism was performed. A metabolic network of primary metabolism in the developing endosperm of barley (Hordeum vulgare), a model plant for temperate cereals, was constructed which includes 257 biochemical and transport reactions across four different compartments. The model was subjected to flux balance analysis (FBA) to study grain yield and metabolic flux distributions in response to oxygen depletion and enzyme deletion. In general, the simulation results were found to be in good agreement with the main biochemical properties of barley seed storage metabolism. The predicted growth rate and the active metabolic pathway patterns under anoxic, hypoxic and aerobic conditions predicted by the model were in accordance with published experimental results. In addition, the model predictions gave insight into the potential role of PPi-metabolism to maintain seed metabolism under oxygen deprivation.




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