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Published on October 29, 2008; 10.1104/pp.108.129817

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Received September 13, 2008
Accepted October 23, 2008

Arabidopsis CLPS3, an Ortholog of Human Polyadenylation Factor CLP1, Functions in Gametophyte, Embryo and Post-Embryonic Development

Denghui Xing , Hongwei Zhao , and Qingshun Quinn Li *

Botany Department, Miami University, Oxford, OH 45056

* Corresponding author; email: liq{at}muohio.edu.

Polyadenylation factor CLP1 is essential for mRNA 3'-end processing in yeast and mammals. The Arabidopsis (Arabidopsis thaliana) CLP1-Similar protein 3 (CLPS3) is an ortholog of human hCLP1. CLPS3 was previously found to be a subunit in the affinity purified PCFS4-TAP complex involved in the alternative polyadenylation of FCA and flowering time control in Arabidopsis. In this study, we further explored the components in the affinity purified CLPS3-TAP complex, from which Arabidopsis cleavage and polyadenylation specificity factor (CPSF) subunits AtCPSF100 and AtCPSF160 were found. This result implies that CLPS3 may bridge CPSF to the PCFS4 complex. Characterization of CLPS3 mutant revealed that CLPS3 was essential for embryo development and important for female gametophyte transmission. Over-expression of CLPS3-TAP fusion caused a range of post-embryonic development abnormalities including early flowering time, altered phyllotaxy, number and shape of flower organs. These phenotypes are associated with the altered gene expression levels of FCA, WUS and CUC1. The decreased ratio of FCA-{beta} to FCA-{gamma} in the over-expression plants suggests that CLPS3 favored the usage of FCA regular poly(A) site over the alternative site. These observations indicate that Arabidopsis CLPS3 might be involved in the processing of pre-mRNAs encoded by a distinct subset of genes that are important in plant development.




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