Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 14, 2008; 10.1104/pp.108.129957

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
149/2/1061    most recent
pp.108.129957v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, M. P.
Right arrow Articles by Ruban, A. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, M. P.
Right arrow Articles by Ruban, A. V.
Agricola
Right arrow Articles by Johnson, M. P.
Right arrow Articles by Ruban, A. V.

Received September 16, 2008
Accepted November 11, 2008

The zeaxanthin-independent and zeaxanthin-dependent qE components of non-photochemical quenching involve common conformational changes within the Photosystem II antenna in Arabidopsis thaliana

Matthew P. Johnson *, Maria L. Perez-Bueno , Ahmad Zia , Peter Horton , and Alexander V. Ruban

School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End, Bancroft Road, London, E1 4NS, United Kingdom; Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, United Kingdom

* Corresponding author; email: m.johnson{at}qmul.ac.uk.

The light harvesting antenna of higher plant photosystem II (LHCII) has the intrinsic capacity to dissipate excess light energy as heat in a process termed nonphotochemical quenching (NPQ). Recent studies suggest that zeaxanthin and lutein both contribute to the rapidly relaxing component of NPQ, qE, possibly acting respectively in the minor monomeric antenna complexes and the major trimeric LHCII. To distinguish whether zeaxanthin and lutein act independently as quenchers at separate sites, or alternatively whether zeaxanthin fulfils an allosteric role regulating lutein-mediated quenching, the kinetics of qE and the qE-related conformational changes ({Delta}A535) were compared in Arabidopsis thaliana mutant/ antisense plants with altered contents of minor antenna (kolhcb6, aslhcb4), trimeric LHCII (aslhcb2), lutein (lut2, lut2npq1, lut2npq2) and zeaxanthin (npq1, npq2). The kinetics of the two components of NPQ induction arising from zeaxanthin-independent and zeaxanthin-dependent qE were both sensitive to changes in the protein composition of the PSII antenna. The replacement of lutein by zeaxanthin or violaxanthin in the internal Lhcb protein binding sites affected the kinetics and relative amplitude of each component, as well as the absolute chlorophyll fluorescence lifetime. Both components of qE were characterized by a conformational change leading to nearly identical absorption changes in the Soret region that indicated the involvement of the LHCII lutein 1 domain. Based on these observations we suggest that both components of qE arise from a common quenching mechanism based upon a conformational change within the PSII antenna, optimized by Lhcb subunit-subunit interactions and tuned by the synergistic effects of external and internally bound xanthophylls.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
F. Passarini, E. Wientjes, R. Hienerwadel, and R. Croce
Molecular Basis of Light Harvesting and Photoprotection in CP24: UNIQUE FEATURES OF THE MOST RECENT ANTENNA COMPLEX
J. Biol. Chem., October 23, 2009; 284(43): 29536 - 29546.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Johnson and A. V. Ruban
Photoprotective Energy Dissipation in Higher Plants Involves Alteration of the Excited State Energy of the Emitting Chlorophyll(s) in the Light Harvesting Antenna II (LHCII)
J. Biol. Chem., August 28, 2009; 284(35): 23592 - 23601.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Li, T. K. Ahn, T. J. Avenson, M. Ballottari, J. A. Cruz, D. M. Kramer, R. Bassi, G. R. Fleming, J. D. Keasling, and K. K. Niyogi
Lutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 Mutant
PLANT CELL, June 1, 2009; 21(6): 1798 - 1812.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists