Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 9, 2009; 10.1104/pp.108.130070

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
149/3/1251    most recent
pp.108.130070v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rippert, P.
Right arrow Articles by Matringe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rippert, P.
Right arrow Articles by Matringe, M.
Agricola
Right arrow Articles by Rippert, P.
Right arrow Articles by Matringe, M.

Received September 19, 2008
Accepted January 5, 2009

Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis

Pascal Rippert , Juliette Puyaubert , Delphine Grisolet , Laure Derrier , and Michel Matringe *

Laboratoire de Physiologie Cellulaire Vegetale, Institut National de la Recherche Agronomique, UMR 1200, 17 Rue des Martyrs F-38054 Grenoble, France; Laboratoire de Physiologie Cellulaire Vegetale, Centre de la Recherche Scientifique, UMR 5168, 17 Rue des Martyrs F-38054 Grenoble, France; Laboratoire de Physiologie Cellulaire Vegetale, Universite Grenoble I, 17 Rue des Martyrs F-38054 Grenoble, France; Laboratoire de Physiologie Cellulaire Vegetale, iRTSV Commissariat a l'Energie Atomique-Grenoble, 17 Rue des Martyrs F-38054 Grenoble, France;

* Corresponding author; email: michel.matringe{at}cea.fr.

While the presence of a complete shikimate pathway within plant plastids is definitively established, the existence of a cytosolic post-chorismate portion of the pathway is still debated. This question is alimented by the presence of a chorismate mutase within the cytosol. Until now, the only known destiny of prephenate, the product of chorismate mutase, is incorporation into tyrosine and/or phenylalanine. Therefore, the presence of a cytosolic chorismate mutase suggests that enzymes involved downstream chorismate mutase in tyrosine or phenylalanine biosynthesis could be present within the cytosol of plant cells. It was thus of particular interest to clarify the subcellular localization of arogenate dehydrogenases (TYRA) and arogenate dehydratases (ADT), which catalyze the ultimate steps in tyrosine and phenylalanine biosynthesis, respectively. The aim of the present study was to address this question in Arabidopsis by analysis the subcellular localization of the two TYRAAt and the six AtADT. The present work excludes the occurrence of a spliced TYRAAt1 transcript encoding a cytosolic TYRA protein. Transient expression analyses of TYRA- and ADT- GFP fusions reveal that the two Arabidopsis TYRA proteins and the six ADT proteins are all targeted within the plastid. Accordingly, TYRA and ADT proteins were both immunodetected in the chloroplast soluble protein fraction (stroma) of Arabidopsis. No TYRA or ADT proteins were immunodetected in the cytosol of Arabidopsis cells. Taken together, all our data exclude the possibility of tyrosine and/or phenylalanine synthesis within the cytosol, at least in green leaves and Arabidopsis cultured cells.




This article has been cited by other articles:


Home page
 Mol PlantHome page
J. Joyard, M. Ferro, C. Masselon, D. Seigneurin-Berny, D. Salvi, J. Garin, and N. Rolland
Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways
Mol Plant, November 12, 2009; (2009) ssp088v1.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists