Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 12, 2008; 10.1104/pp.108.130450

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
149/3/1354    most recent
pp.108.130450v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruggenthaler, P.
Right arrow Articles by Waigmann, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruggenthaler, P.
Right arrow Articles by Waigmann, E.
Agricola
Right arrow Articles by Ruggenthaler, P.
Right arrow Articles by Waigmann, E.

Received September 26, 2008
Accepted November 25, 2008

Microtubules associated protein AtMPB2C plays a role in organization of cortical microtubules, stomata patterning and tobamovirus infectivity

Pia Ruggenthaler , Daniela Fichtenbauer , Julia Krasensky , Claudia Jonak , and Elisabeth Waigmann *

Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Medical Biochemistry, Medical University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria; Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, 1030 Vienna, Austria

* Corresponding author; email: elisabeth.waigmann{at}meduniwien.ac.at.

AtMPB2C is the Arabidopsis thaliana homologue of MPB2C, a microtubule associated host factor of tobacco mosaic virus movement protein that has been previously identified in Nicotiana tabacum. To analyze the endogenous function of AtMPB2C and its role in viral infections, transgenic Arabidopsis plant lines stably overexpressing GFP-AtMPB2C were established. The GFP-AtMPB2C fusion protein was detectable in various cell types and organs and localized at microtubules in a punctuate pattern or in filaments. To determine, whether overexpression impacted on cortical microtubular cytoskeleton, GFP-AtMPB2C overexpressing plants were compared to known microtubular marker lines. In rapidly elongated cell types such as vein cells and root cells, GFP-AtMPB2C overexpression caused highly unordered assemblies of cortical microtubules, a disturbed, snake-like microtubular shape and star-like crossing points of microtubules. Phenotypically, GFP-AtMPB2C transgenic plants showed retarded growth but were viable and fertile. Seedlings of GFP-AtMPB2C transgenic plants were characterized by clockwise twisted leaves, clustered stomata and enhanced drought tolerance. GFP-AtMPB2C overexpressing plants showed increased resistance against oilseed rape mosaic virus, a close relative of tobacco mosaic virus, but not against cucumber mosaic virus when compared to Arabidopsis wild type plants. These results suggest that AtMPB2C is involved in the alignment of cortical microtubules, the patterning of stomata and in restricting tobamoviral infections.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists