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Published on November 26, 2008; 10.1104/pp.108.131144

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Received October 11, 2008
Accepted November 22, 2008

Identification of an apoplastic protein that involved in initial phase of salt stress response in rice root by two-dimensional electrophoresis approach

Lei Zhang , Li-Hong Tian , Jun-Feng Zhao , Yun Song , Cui-Jun Zhang , and Yi Guo *

Institute of Molecular and Cell Biology, Hebei Normal University, Shijiazhuang 050016, China

* Corresponding author; email: guoyi{at}mail.hebtu.edu.cn.

The apoplast of plant cells, which carries out multiple functions in plant metabolism and signaling, is not only a barrier but also the linker between the environment and the protoplast. To investigate the role of apoplastic proteins in the salt-stress response, 10-day-old rice plants were treated with 200 mM NaCl for 1, 3 or 6 hours, and the soluble apoplast proteins were extracted for differential analysis compared to untreated controls using two-dimensional electrophoresis. Ten protein spots that increased or decreased significantly in abundance were identified by mass spectrometry. These proteins included some well-known biotic and abiotic stress-related proteins. Among them, an apoplastic protein, with extracellular-domain-like cysteine-rich motifs (DUF26), Oryza sativa root meander curling (OsRMC) has shown drastically increased abundance in response to salt stress during the initial phase. OsRMC RNAi transgenic rice has been generated to assess the function of OsRMC in the salt-stress response. The results show that knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition and improved salt-stress tolerance to NaCl than untransgenic plants. These results indicate that plant apoplastic proteins may have important roles in the plant salt-stress response.




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K. Witzel, A. Weidner, G.-K. Surabhi, A. Borner, and H.-P. Mock
Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity
J. Exp. Bot., August 1, 2009; 60(12): 3545 - 3557.
[Abstract] [Full Text] [PDF]


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