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Published on December 3, 2008; 10.1104/pp.108.131573

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Received October 22, 2008
Accepted November 28, 2008

The Analysis of the Nicotiana Tabacum (L.) Stigma/Style Transcriptome Reveals Gene Expression Differences between Wet and Dry Stigma Species

Andrea C. Quiapim , Michael S. Brito , Luciano A. S. Bernardes , Idalete daSilva , Iran Malavazi , Henrique C. DePaoli , Jeanne B. Molfetta-Machado , Silvana Guiliatti , Gustavo H. Goldman , and Maria Helena S. Goldman *

Departamento de Biologia, Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Brazil; Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Brazil; PPG Genetica e Melhoramento de Plantas, Faculdade de Ciencias Agrarias e Veterinarias, UNESP, Brazil; Departamento de C. Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Brazil

* Corresponding author; email: mgoldman{at}ffclrp.usp.br.

The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Nicotiana tabacum represents a model of wet stigma and its stigmas/styles express genes to accomplish the appropriate functions. For a large scale study of gene expression during tobacco pistil development and preparation for pollination, we generated 11,216 high-quality ESTs from stigmas/styles and created the TOBEST database. These ESTs were assembled in 6,177 clusters, from which 52.1% are pistil transcripts/genes of unknown function. The 21 clusters with the highest number of ESTs (putative higher expression levels) correspond to genes associated with defense mechanisms or pollen-pistil interactions. The database analysis unraveled tobacco sequences homologous to the Arabidopsis genes involved in specifying pistil identity or determining normal pistil morphology and function. Additionally, 782 independent clusters were examined by macroarray, revealing 46 stigma/style preferentially expressed genes. Real time RT-PCR experiments validated the pistil-preferential expression for nine out of ten genes tested. A search for these 46 genes in the Arabidopsis pistil datasets demonstrated that only 11 sequences, with putative equivalent molecular functions, are expressed in this dry stigma species. The reverse search for the Arabidopsis pistil genes in the TOBEST exposed a partial overlap between these dry and wet stigma transcriptomes. The TOBEST represents the most extensive survey of gene expression in the stigmas/styles of wet stigma plants and our results indicate that wet and dry stigmas/styles express common as well as distinct genes in preparation for the pollination process.




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