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Published on February 11, 2009; 10.1104/pp.108.131714

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Received October 25, 2008
Accepted January 30, 2009

Exploring the Mechanism of Physcomitrella patens Desiccation Tolerance through a Proteomic Strategy

Xiao Qin Wang , Ping Fang Yang , Zheng Liu , Wei Zhong Liu , Yong Hu , Hui Chen , Ting Yun Kuang , Zhen Ming Pei , Shi Hua Shen , and Yi Kun He *

College of Life Sciences, Capital Normal University, Beijing 100037, China; Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, USA; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, UK CB2 3EA; Department of Biology, Duke University, Durham, NC 27708, USA

* Corresponding author; email: yhe{at}mail.cnu.edu.cn.

The moss, Physcomitrella patens, has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over one month to 10% of their original fresh weight. We report that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling, and microtubule depolymerization. Despite the severe desiccation, the membrane system maintains integrity. Through two-dimensional gel electrophoresis (2-DE) and image analysis, we identified 71 proteins as desiccation-responsive. Following identification and functional categorization, we found that a majority of the desiccation-responsive proteins were involved in metabolism, cytoskeleton, defense, and signaling. Degradation of cytoskeletal proteins might result in the cytoskeletal disassembly and consequent changes in the cell structure. Late embryogenesis abundant (LEA) proteins and reactive oxygen species-scavenging enzymes are both prominently induced and they might help to diminish the damage brought by desiccation.






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