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Published on December 19, 2008; 10.1104/pp.108.131862

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Received October 29, 2008
Accepted December 16, 2008

Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in Vitis vinifera L. and suggests additional targets in the pathway

Nancy Terrier *, Laurent Torregrosa , Agnes Ageorges , Sandrine Vialet , Clotilde Verries , Veronique Cheynier , and Charles Romieu

UMR SPO 1083, Campus SUPAGRO-INRA 2 Place Viala, F-34060 Montpellier, France; UMR DIAPC 1097, Campus SUPAGRO-INRA, 2, Place Viala, F-34060 Montpellier, France

* Corresponding author; email: terrier{at}supagro.inra.fr.

Grapevine proanthocyanidins contribute to plant defence mechanisms against biotic stress and also play a critical role in organoleptic properties of wine. In grapevine berry, these compounds are mainly accumulated in exocarps and seeds in the very early stages of development. A previous study has already identified VvMybPA1 (Bogs et al., 2007) as a first transcription factor involved in the regulation of proanthocyanidin pathway along seed development in grapevine. A novel Myb factor VvMybPA2, which is described in this study, is in contrast mainly expressed in the exocarp of young berries and in the leaves. This transcription factor shows very high protein sequence homology with other plant Myb factors which regulate flavonoid biosynthesis. Ectopic expression of either VvMybPA1 or VvMybPA2 in Vitis vinifera hairy roots induced qualitative and quantitative changes of the proanthocyanidin profiles. High throughput transcriptomic analyses of transformed grapevine organs identified a large set of putative targets of the VvMybPA1 and VvMybPA2 transcription factors. Both genes significantly activated enzymes of the flavonoid pathway, including anthocyanidin reductase and leucoanthocyanidin reductase 1, the specific terminal steps in the biosynthesis of respectively epicatechin and catechin, but not leucoanthocyanidin reductase 2. The functional annotation of the genes whose expression was modified revealed putative new actors of the proanthocyanidin pathway, such as glucosyltransferases and transporters.




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