Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 13, 2009; 10.1104/pp.108.131979

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
150/1/308    most recent
pp.108.131979v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamaguchi, T.
Right arrow Articles by Shibuya, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamaguchi, T.
Right arrow Articles by Shibuya, N.
Agricola
Right arrow Articles by Yamaguchi, T.
Right arrow Articles by Shibuya, N.

Received November 25, 2008
Accepted March 11, 2009

Suppression of a phospholipase D gene, OsPLD, activates defense responses and increases disease resistance in rice

Takeshi Yamaguchi *, Masaharu Kuroda , Hiromoto Yamakawa , Taketo Ashizawa , Kazuyuki Hirayae , Leona Kurimoto , Tomonori Shinya , and Naoto Shibuya

National Agricultural Research Center, Joetsu, Niigata 943-0193, Japan; Department of Life Sciences, Faculty of Agriculture, Meiji University, Kawasaki, Kanagawa 214-8571, Japan

* Corresponding author; email: tkyama{at}affrc.go.jp.

Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of rice genome database indicated the presence of 17 PLD genes in the genome, among which OsPLD{alpha}1, OsPLD{alpha}5, and OsPLD{beta}1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown plants for each PLD isoform by introducing gene-specific RNAi constructs. One of them, OsPLD{beta}1-knockdown plants showed the accumulation of reactive oxygen species in the absence of pathogen infection. RT-PCR and DNA microarray analyses revealed that the knockdown of OsPLD{beta}1 resulted in the up-/down-regulation of more than 1,400 genes, including the induction of defense-related genes such as PR protein genes and WRKY/ERF family transcription factor genes. HR-like cell death and phytoalexin production were also observed at a later phase of growth in the OsPLD{beta}1-knockdown plants. These results indicated that the OsPLD{beta}1-knockdown plants spontaneously activate the defense responses in the absence of pathogen infection. Furthermore, the OsPLD{beta}1-knockdown plants exhibited increased resistance to the infection of major pathogens of rice, Pyricularia grisea and Xanthomonas oryzae pv. oryzae. These results suggested that OsPLD{beta}1 functions as a negative regulator of defense responses and disease resistance in rice.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists