Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 23, 2009; 10.1104/pp.108.132134

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
149/3/1341    most recent
pp.108.132134v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kikuchi, R.
Right arrow Articles by Handa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kikuchi, R.
Right arrow Articles by Handa, H.
Agricola
Right arrow Articles by Kikuchi, R.
Right arrow Articles by Handa, H.

Received November 4, 2008
Accepted January 21, 2009

Molecular and functional characterization of PEBP genes in barley (Hordeum vulgare L.) reveal the diversification of their roles in flowering

Rie Kikuchi , Hiroyuki Kawahigashi , Tsuyu Ando , Takuji Tonooka , and Hirokazu Handa *

Plant Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan; Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba 305-0854, Japan; Barley Research Subteam, National Institute of Crop Science, Tsukuba 305-8518, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan

* Corresponding author; email: hirokazu{at}affrc.go.jp.

Five barley PEBP genes were analyzed to clarify their functional roles in flowering using transgenic, expression and QTL analyses. Introduction of HvTFL1 and HvMFT1 into rice plants did not result in any changes in flowering, suggesting that these two genes have functions distinct from flowering. Overexpression of HvFT1, HvFT2 and HvFT3 in rice resulted in early heading, indicating that these FT-like genes can act as promoters of the floral transition. HvFT1 transgenic plants showed the most robust flowering initiation. In barley HvFT1 was expressed at the time of shoot meristem phase transition. These results suggest that HvFT1 is the key gene responsible for flowering in the barley FT-like gene family. HvFT2 transgenic plants also showed robust flowering initiation, but HvFT2 was expressed only under short-day (SD) conditions during the phase transition, suggesting that its role is limited to specific photoperiodic conditions in barley. Flowering activity in HvFT3 transgenic rice was not as strong and was modulated by the photoperiod. These results suggest that HvFT3 functions in flowering promotion, but that its effect is indirect. HvFT3 expression was observed in Morex, a barley cultivar carrying a dominant allele of Ppd-H2, a major QTL for flowering under SD conditions, although no expression was detected in Steptoe, a cultivar carrying ppd-H2. HvFT3 was expressed in Morex under both long-day (LD) and SD conditions, although its expression was incleased under SD conditions. HvFT3 was mapped to chromosome 1HL, the same chromosome that carries Ppd-H2. Genomic sequence analyses revealed that Morex possesses an intact HvFT3 gene, whereas most of this gene has been lost in Steptoe. These data strongly suggest that HvFT3 may be identical to Ppd-H2.




This article has been cited by other articles:


Home page
 Plant CellHome page
C. Alonso-Blanco, M. G.M. Aarts, L. Bentsink, J. J.B. Keurentjes, M. Reymond, D. Vreugdenhil, and M. Koornneef
What Has Natural Variation Taught Us about Plant Development, Physiology, and Adaptation?
PLANT CELL, July 1, 2009; 21(7): 1877 - 1896.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists