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Published on December 3, 2008; 10.1104/pp.108.132217

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Received November 6, 2008
Accepted November 28, 2008

Wheat Cryptochromes: Subcellular Localization and Involvement in Photomorphogenesis and Osmotic Stress Responses

Pei Xu , Yang Xiang , Huilan Zhu , Haibin Xu , Zhengzhi Zhang , Caiqin Zhang , Lixia Zhang , and Zhengqiang Ma *

The Applied Plant Genomics Lab, Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Jiangsu 210095, China; Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

* Corresponding author; email: zqm2{at}njau.edu.cn.

Cryptochromes (CRYs) are blue light receptors important for plant growth and development. Comprehensive information on monocot CRYs is currently only available for rice. We report here the molecular and functional characterization of two CRY genes, TaCRY1a and TaCRY2, from the monocot wheat. The expression of TaCRY1a was most abundant in seedling leaves and barely detected in roots and germinating embryos under normal growth conditions. The expression of TaCRY2 in germinating embryos was equivalent to that in leaves and much higher than the TaCRY1a counterpart. Transition from dark to light slightly affected the expression of TaCRY1a and TaCRY2 in leaves, and red light produced a stronger induction of TaCRY1a. Treatment of seedlings with high salt, PEG and ABA up-regulated TaCRY2 in roots and germinating embryos. TaCRY1a displays a light-responsive nucleocytoplasmic shuttling pattern similar to that of Arabidopsis CRY1, contains nuclear localization domains in both the N and C termini and includes information for nuclear export in its N-terminal domain. TaCRY2 was localized to the nucleus in the dark. Expression of TaCRY1a-GFP or TaCRY2-GFP in Arabidopsis conferred a shorter hypocotyl phenotype under blue light. These transgenic Arabidopsis plants showed higher sensitivity to high salt, osmotic stress and ABA treatment during germination and post-germination development, and they displayed altered expression of stress/ABA responsive genes. The primary root growth in transgenic seedlings was less tolerant of ABA. These observations indicate that TaCRY1 and TaCRY2 might be involved in the ABA signaling pathway in addition to their role in primary blue light signal transduction.




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M. Liedvogel and H. Mouritsen
Cryptochromes--a potential magnetoreceptor: what do we know and what do we want to know?
J R Soc Interface, November 11, 2009; (2009) rsif.2009.0411.focusv1.
[Abstract] [Full Text] [PDF]


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