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Published on January 7, 2009; 10.1104/pp.108.132688

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Received November 16, 2008
Accepted December 31, 2008

Compromised Virus-induced Gene Silencing in RDR6-deficient Plants

Fabian E. Vaistij * and Louise Jones

Centre for Novel Agricultural Products; Department of Biology, University of York, York YO10 5DD, United Kingdom

* Corresponding author; email: fv4{at}york.ac.uk.

RNA silencing in plants serves as a potent anti-viral defence mechanism through the action of small interfering RNA (siRNAs) which direct RNA degradation. siRNAs can be derived directly from the viral genome or via the action of host-encoded RNA-dependent RNA polymerases (RDRs). Plant genomes encode for multiple RDRs and it has been demonstrated that plants defective for RDR6 hyper-accumulate several classes of virus. In this study we compared the effectiveness of virus-induced gene silencing (VIGS) and RNA-directed DNA methylation (RdDM) in wild-type and RDR6-deficient Nicotiana benthamiana plants. For the potexvirus potato virus X (PVX) and the potyvirus Plum Pox Virus (PPV) efficiency of both VIGS and RdDM were compromised in RDR6-defective plants despite accumulating high levels of viral siRNAs similar to infection of wild-type plants. The reduced efficiency of VIGS and RdDM was unrelated to the size class of siRNA produced and, at least for PVX, was not dependent the presence of the virus-encoded silencing suppressor protein, 25K. We suggest that primary siRNAs produced from PVX and PPV in the absence of RDR6 may not be good effectors of silencing and that RDR6 is required to produce secondary siRNAs that drive a more effective anti-viral response.






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