Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 7, 2009; 10.1104/pp.108.133405

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
149/3/1325    most recent
pp.108.133405v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dam, S.
Right arrow Articles by Stougaard, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dam, S.
Right arrow Articles by Stougaard, J.
Agricola
Right arrow Articles by Dam, S.
Right arrow Articles by Stougaard, J.

Received November 28, 2008
Accepted January 3, 2009

The proteome of seed development in the model legume Lotus japonicus

Svend Dam , Brian S. Laursen , Jane H. Ornfelt , Bjarne Jochimsen , Hans Henrik Stærfeldt , Carsten Friis , Kasper Nielsen , Nicolas Goffard , Soren Besenbacher , Lene Krusell , Shusei Sato , Satoshi Tabata , Ida B. Thogersen , Jan J. Enghild , and Jens Stougaard *

Centre for Carbohydrate Recognition and Signalling; Center for Insoluble Protein Structures (inSPIN); Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark; Center for Biological Sequence Analysis, Technical University of Denmark, Anker Engelundsvej 1, DK-2800 Kgs Lyngby, Denmark; Institut Louis Malarde, GP Box 30, 98713 Papeete Tahiti, French Polynesia; ARC Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia; Bioinformatics Research Center, University of Aarhus, C. F. Mollers Alle, Building 1110, DK-8000 Aarhus, Denmark; Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818, Japan

* Corresponding author; email: stougaard{at}mb.au.dk.

We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean and pea, Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined and this indicates that the composition of mature Lotus seed is more similar to soybean than pea. In a first attempt to determine the seed proteome both a 2-D PAGE approach and a GeLC-MS/MS approach were used. Globulins were analyzed by 2-D PAGE and five legumins, LLP1-5, and two convicilins, LCP1-2, were identified by MALDI Q-TOF mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a GeLC-MS/MS approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases respectively. All the proteome data including the experimental data and MS spectra peaks were collected in a database that is available to the scientific community via a web-interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry and regulation of seed development in Lotus. Together with a new a web-interface (http://bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Thompson, J. Burstin, and K. Gallardo
Post-Genomics Studies of Developmental Processes in Legume Seeds
Plant Physiology, November 1, 2009; 151(3): 1023 - 1029.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. Matamoros, J. Loscos, K.-J. Dietz, P. M. Aparicio-Tejo, and M. Becana
Function of antioxidant enzymes and metabolites during maturation of pea fruits
J. Exp. Bot., October 11, 2009; (2009) erp285v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Goffard, T. Frickey, and G. Weiller
PathExpress update: the enzyme neighbourhood method of associating gene-expression data with metabolic pathways
Nucleic Acids Res., July 1, 2009; 37(suppl_2): W335 - W339.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists