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Published on March 11, 2009; 10.1104/pp.108.133454

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Received December 1, 2008
Accepted March 7, 2009

Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes

Qibin Ma , Xiaoyan Dai , Yunyuan Xu , Jing Guo , Yaju Liu , Na Chen , Jun Xiao , Dajian Zhang , Zhihong Xu , Xiansheng Zhang , and Kang Chong *

Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China; the National Center for Plant Gene Research, Beijing 100093, China

* Corresponding author; email: chongk{at}ibcas.ac.cn.

MYB transcription factors play central roles in plant response to abiotic stresses. How stress affects development is poorly understood. Here, we show that OsMYB3R-2 functions in both stress and developmental processes in rice. Transgenic plants overexpressing OsMYB3R-2 exhibited enhanced cold tolerance. Cold treatment greatly induced the expression of OsMYB3R-2, which encodes an active transcription factor. We showed that OsMYB3R-2 specifically bound to mitosis-specific activators (MSA) cis-element (T/C)C(T/C)AACGG(T/C)(T/C)A, a conserved sequence that was found in promoters of cyclin genes such as OsCycB1;1 and OsKNOLLE2. In addition, overexpression of OsMYB3R-2 in rice led to higher transcript levels of several G2/M phase-specific genes, including OsCycB1;1, OsCycB2;1, OsCycB2;2, OsCDC20.1, than those in OsMYB3R-2 antisense lines or wild type plants in response to cold treatments. Flow cytometry analysis revealed an increased cell mitotic index in overexpressed transgenic lines of OsMYB3R-2 after cold treatment. Furthermore, resistance to cold stress in the transgenic plants overexpressing OsCycB1;1 was also enhanced. The level of cellular free proline was increased in the overexpressed rice lines of OsMYB3R-2 and OsCycB1;1 transgenic plants compared with wild type under the cold treatment. These results suggest that OsMYB3R-2 target OsCycB1;1 and regulate the progress of the cell cycle during chilling stress. OsCPT1, which may be involved in the dehydration responsive-element binding factor 1A (DREB1A) pathway, showed the same transcription pattern in response to cold as did OsCycB1;1 in transgenic rice. Therefore, a cold resistance mechanism in rice could be mediated by regulating cell cycle, which is controlled by key genes including OsMYB3R-2.






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