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Published on February 6, 2009; 10.1104/pp.108.133819

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Received December 9, 2008
Accepted February 3, 2009

The short-rooted phenotype of the brevis radix mutant partly reflects root ABA hypersensitivity

Americo Rodrigues , Julia Santiago , Silvia Rubio , Angela Saez , Karen S. Osmont , Jose Gadea , Christian S. Hardtke , and Pedro L. Rodriguez *

Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas, Avd de los Naranjos, E-46022 Valencia, Spain; Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015 Lausanne, Switzerland

* Corresponding author; email: prodriguez{at}ibmcp.upv.es.

To gain further insight into ABA signaling and its role in growth regulation, we have screened for Arabidopsis thaliana mutants hypersensitive to ABA-mediated root growth inhibition. As a result, we have identified a loss-of-function allele of BREVIS RADIX (BRX) in Columbia background, named brx-2, which shows enhanced response to ABA-mediated inhibition of root growth. BRX encodes a key regulator of cell proliferation and elongation in the root, which has been implicated in the brassinosteroid (BR) pathway as well as regulation of auxin-responsive gene expression. Mutants affected in BR signaling that are not impaired in root growth, such as bes1-D, bzr1-D and bsu1-D, also showed enhanced sensitivity to ABA-mediated inhibition of root growth. Triple loss-of-function mutants affected in PP2Cs that act as negative regulators of ABA signaling showed impaired root growth in the absence of exogenous ABA, indicating that disturbed regulation of ABA sensitivity impairs root growth. In agreement with this result, diminishing ABA-sensitivity of brx-2 by crossing it with a 35S:HAB1 ABA-insensitive line allowed significantly higher recovery of root growth after BL treatment. Finally, transcriptomic analysis revealed that ABA treatment negatively affects auxin signaling in wt and brx-2 roots and that ABA response is globally altered in brx-2. Taken together, our results reveal an interaction between BRs, auxin and ABA in the control of root growth and indicate that altered sensitivity to ABA is partly responsible for the brx short root phenotype.




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