Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 15, 2009; 10.1104/pp.108.134601

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrowOA All Versions of this Article:
150/2/825    most recent
pp.108.134601v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Husted, S.
Right arrow Articles by Jensen, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Husted, S.
Right arrow Articles by Jensen, P. E.
Agricola
Right arrow Articles by Husted, S.
Right arrow Articles by Jensen, P. E.

Received December 18, 2008
Accepted April 4, 2009

Manganese deficiency leads to genotype specific changes in fluorescence induction kinetics and state transitions

Soren Husted *, Kristian H. Laursen , Christopher A. Hebbern , Sidsel B. Schmidt , Pai Pedas , Anna Haldrup , and Poul E. Jensen

Plant and Soil Science Laboratory, Department of Agriculture and Ecology; VKR Research Centre "Pro-Active Plants", Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark

* Corresponding author; email: shu{at}life.ku.dk.

Barley genotypes display a marked difference in their ability to tolerate growth at low manganese (Mn) concentrations, a phenomenon designated as differential Mn efficiency. Induction of Mn deficiency in two genotypes differing in Mn efficiency led to a decline in the quantum yield efficiency (Fv/Fm) for both, however, faster in the Mn-inefficient genotype. Leaf tissue and thylakoid Mn concentrations were reduced under Mn deficiency, but no difference between genotypes was observed and no visual Mn deficiency symptoms were developed. Analysis of the fluorescence induction kinetics (FIK) revealed that in addition to the usual O-J-I-P steps, clear K- and D-steps were developed in the Mn-inefficient genotype under Mn deficiency. These marked changes indicated damages to photosystem II (PSII). This was further substantiated by state transition measurements, indicating that the ability of plants to redistribute excitation energy was reduced. The percent change in state transitions for control plants with normal Mn supply of both genotypes were 9-11%. However, in Mn deficient leaves of the Mn inefficient genotypes, state transitions were reduced to less than 1%, whereas no change was observed for the Mn-efficient genotypes. Immunoblotting and the Chl a/b ratio confirmed that Mn deficiency in general resulted in a significant reduction in abundance of PSII reaction centers (RC) relative to the peripheral antenna. In addition, PSII appeared to be significantly more affected by Mn limitation than PSI. However, the striking genotypic differences observed in Mn deficient plants, when analyzing state transitions and FIK, could not be correlated with specific changes in photosystem proteins. Thus, there is no simple linkage between protein expression and the differential reduction in state transition and FIK observed for the genotypes under Mn deficiency.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists