Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 11, 2009; 10.1104/pp.108.134882

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
150/1/27    most recent
pp.108.134882v2
pp.108.134882v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, M.
Right arrow Articles by Thelen, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, M.
Right arrow Articles by Thelen, J. J.
Agricola
Right arrow Articles by Chen, M.
Right arrow Articles by Thelen, J. J.

Received December 30, 2008
Accepted February 27, 2009

System Analysis of An Arabidopsis Mutant Altered in de novo Fatty Acid Synthesis Reveals Diverse Changes in Seed Composition and Metabolism

Mingjie Chen , Brian P. Mooney , Martin Hajduch , Trupti Joshi , Mingyi Zhou , Dong Xu , and Jay J. Thelen *

Interdisciplinary Plant Group and Division of Biochemistry; Computer Science Department; DNA Core Microarray Facility; Charles Gehrke Proteomics Center 109 Christopher S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, Missouri 65211, USA

* Corresponding author; email: thelenj{at}missouri.edu.

Embryo-specific overexpression of biotin carboxyl carrier protein 2 (BCCP2) inhibited plastid acetyl-CoA carboxylase (ACCase) resulting in altered oil, protein, and carbohydrate composition in mature Arabidopsis seed. To characterize gene and protein regulatory consequences of this mutation, global microarray, two-dimensional difference gel electrophoresis, iTRAQ, and quantitative immunoblotting were performed in parallel. These analyses revealed: 1) transgenic overexpression of BCCP2, did not affect expression of other ACCase subunits; 2) four subunits to plastid pyruvate dehydrogenase complex (PDC) were 25-70% down-regulated at protein but not transcript levels; 3) key glycolysis and de novo fatty acid/lipid synthesis enzymes were induced; 4) multiple storage proteins, but not cognate transcripts, were up-regulated; and 5) biotin synthesis pathway was up-regulated at both transcript and protein levels. Biotin production appears closely matched to endogenous BCCP levels, since overexpression of BCCP2 produced mostly apo-BCCP2 and the resulting ACCase-compromised, low-oil phenotype. Differential expression of glycolysis, plastid PDC, fatty acid, and lipid synthesis activities indicate multiple, complex regulatory responses including feedback as well as futile "feed-forward" elicitation in the case of fatty acid and lipid biosynthetic enzymes. Induction of storage proteins reveals oil and protein synthesis share carbon intermediate(s) and that reducing malonyl-CoA flow into fatty acids diverts carbon into amino acid and protein synthesis.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
N. L. Houston, M. Hajduch, and J. J. Thelen
Quantitative Proteomics of Seed Filling in Castor: Comparison with Soybean and Rapeseed Reveals Differences between Photosynthetic and Nonphotosynthetic Seed Metabolism
Plant Physiology, October 1, 2009; 151(2): 857 - 868.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists