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Published on January 28, 2009; 10.1104/pp.108.135160

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Received January 1, 2009
Accepted January 23, 2009

The temperature sensitive brush mutant of the legume Lotus japonicus reveals a link between root development and nodule infection by rhizobia

Makoto Maekawa-Yoshikawa , Judith Muller , Naoya Takeda , Takaki Maekawa , Shusei Sato , Satoshi Tabata , Andreas Brachmann , and Martin Parniske *

University of Munich, Faculty of Biology, Genetics, Großhaderner Str. 2-4, 82152 Munchen-Martinsried, Germany; Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan

* Corresponding author; email: parniske{at}lmu.de.

The brush mutant of Lotus japonicus exhibits a temperature-dependent impairment in nodule, root, and shoot development. At 26°C brush formed fewer nodules, most of which were not colonized by rhizobia bacteria. Primary root growth was retarded and the anatomy of the brush root apical meristem revealed a distorted cellular organization and reduced cell expansion. Reciprocal grafting of brush with wild type plants indicated that this genotype only affected the root and that the shoot phenotype was a secondary effect. The root and nodulation phenotype co-segregated as a single Mendelian trait and the BRUSH gene could be mapped to the short arm of chromosome 2. At 18°C, the brush root anatomy was rescued and similar to the wild type, and primary root length, number of infection threads, and nodule formation were partially rescued. Superficially, the brush root phenotype resembled the ethylene-related ‘thick short root’ syndrome. However, treatment with ethylene inhibitor did not recover the observed phenotypes, although brush primary roots were slightly longer. The defects of brush in root architecture and infection thread development, together with an intact nodule architecture and the complete absence of symptoms from shoots, suggest that BRUSH affects cellular differentiation in a tissue dependent way.




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J. Perry, A. Brachmann, T. Welham, A. Binder, M. Charpentier, M. Groth, K. Haage, K. Markmann, T. L. Wang, and M. Parniske
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[Abstract] [Full Text] [PDF]


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