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Published on April 10, 2009; 10.1104/pp.109.135368

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Received January 7, 2009
Accepted April 6, 2009

Arabidopsis CHLI2 can substitute for CHLI1

Yi-Shiuan Huang and Hsou-min Li *

Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan

* Corresponding author; email: mbhmli{at}gate.sinica.edu.tw.

The I subunit of Mg-chelatase is encoded by two genes in Arabidopsis, CHLI1 and CHLI2. Conflicting results have been reported concerning the functions of the two proteins. We show here that the chli1/chli1 chli2/chli2 double-knockout mutant was albino. Comparison with the pale-green phenotype of a chli1/chli1 single-knockout mutant indicates that CHLI2 could support some chlorophyll biosynthesis in the complete absence of CHLI1. Real-time quantitative RT-PCR showed that CHLI2 was expressed at a much lower level than CHLI1. The chli1/chli1 chli2/chli2 double mutant could be fully rescued by expressing a transgene of CHLI2 driven by the CHLI1 promoter. These results suggest that differences between CHLI1 and CHLI2 lie mostly in their expression levels. Furthermore, both the chli1/chli1 and the chli2/chli2 single-knockout mutants had lower survival rates during de-etiolation than the wild type, suggesting that both genes are required for optimal growth during de-etiolation. In addition, we show that a semi-dominant chli1 mutant allele and the chli1/chli1 chli2/chli2 double mutant accumulated Lhcb1 transcripts when treated with the herbicide norflurazon, indicating that knocking out the CHLI activity causes the genome-uncoupled (gun) phenotype.






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