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Published on April 17, 2009; 10.1104/pp.109.135418

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Received January 8, 2009
Accepted April 14, 2009

Disruption of OsYSL15 leads to iron inefficiency in rice plants

Sichul Lee , Jeff C. Chiecko , Sun A Kim , Elsbeth L. Walker , Youngsook Lee , Mary Lou Guerinot , and Gynheung An *

Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea; Department of Biology, University of Massachusetts, Amherst, MA 01003, USA; Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, USA 03755

* Corresponding author; email: genean{at}postech.ac.kr.

Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice YS1-like (YSL) genes that are strongly induced by iron deficiency. The OsYSL15 promoter fusion to GUS showed that it was expressed in all root tissues when iron was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15: green fluorescent protein fusion was localized to the plasma membrane. OsYSL15 functionally complemented yeast strains defective in iron uptake on media containing Fe3+-deoxymugineic acid and Fe2+-nicotianamine.Two insertional osysl15 mutants exhibited chlorotic phenotypes under iron deficiency and had reduced Fe concentrations in their shoots, roots, and seeds. Nitric oxide treatment reversed this chlorosis under Fe-limiting conditions. Overexpression of OsYSL15 increased the iron concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in iron uptake and distribution in rice plants.






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