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Published on April 29, 2009; 10.1104/pp.109.136036

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Received January 25, 2009
Accepted April 26, 2009

Sucrose control of translation mediated by a uORF encoded peptide

Fatemeh Rahmani , Maureen Hummel , Jolanda Schuurmans , Anika Wiese-Klinkenberg , Sjef Smeekens , and Johannes Hanson *

Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Centre for BioSystems Genomics, POB 98, 6700 AB, Wageningen, The Netherlands

* Corresponding author; email: s.j.hanson{at}uu.nl.

Regulation of gene expression through translational control is common in many organisms. The Arabidopsis transcription factor bZIP11 is translational repressed in response to sucrose resulting in sucrose regulated changes in amino acid metabolism. The 5'leader of the bZIP11 mRNA harbors several upstream open reading frames (uORFs), of which the second uORF (uORF2) is well conserved among bZIP11 homologous genes. The uORF2 element encodes a sucrose control peptide (SC-peptide) of 28 residues that is sufficient for imposing sucrose induced repression of translation (SIRT) on a hetrologous mRNA. Detailed analysis of the SC-peptide suggests that it functions as an attenuator peptide. Results suggest that the SC-peptide inhibits bZIP11 translation in response to high sucrose levels by stalling the ribosome on the mRNA. The conserved non-canonical AUG contexts of bZIP11 uORFs allow inefficient translational initiation of the uORF, resulting in translation initiation of the scanning ribosome at the AUG codon of the bZIP11 main open reading frame. The results presented show that sucrose dependent signaling mediates differential translation of mRNAs containing SC-peptides encoding uORFs.






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