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Published on May 15, 2009; 10.1104/pp.109.136382

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Received January 29, 2009
Accepted May 12, 2009

The organization pattern of root border-like cells of Arabidopsis thaliana is dependent on cell wall homogalacturonan

Caroline Durand , Maate Vicre-Gibouin , Marie Laure Follet-Gueye , Ludovic Duponchel , Myriam Moreau , Patrice Lerouge , and Azeddine Driouich *

Laboratoire Glyco-MEV, UPRESA 4358, IFRMP 23, Plate-forme de Recherche en Imagerie Cellulaire de Haute Normandie, Universite de Rouen, 76821 Mont Saint Aignan. France; UMR CNRS 8516, LASIR, Laboratoire de Spectrochimie Infrarouge et Raman, Universite de Lille, 59655 Villeneuve d'Ascq Cedex. France

* Corresponding author; email: azeddine.driouich{at}univ-rouen.fr.

Border-like cells are released by Arabidopsis thaliana root tips as organized layers of several cells that remain attached to each other rather than completely detached from each other as usually observed in border cells of many species (Vicré et al., 2005. Plant Physiol. 138: 998-1008). Unlike border cells, cell attachment between border-like cells is maintained after their release into the external environment. To investigate the role of cell wall polysaccharides in the attachment and organization of border-like cells, we have examined their release in several well-characterized mutants defective in the biosynthesis of xyloglucan, cellulose or pectin. Our data show that among all mutants examined, only quasimodo mutants (qua1-1 and qua2-1), that have been characterized as producing less homogalacturonan, had an altered border-like cells phenotype as compared to wild type. Border-like cells in both lines were released as isolated cells separated from each other with the phenotype being much more pronounced in qua1-1 than in qua2-1. Further analysis of border-like cells in qua1-1 mutant using immunocytochemistry and a set of anti-cell wall polysaccharide antibodies showed that the loss of the wild-type phenotype was accompanied by i) a reduction in homogalacturonan-JIM5 epitope in the cell wall of border-like cells confirmed by Fourier Transformed Infrared microspectrometry and ii) the secretion of an abundant mucilage that is enriched in xylogalacturonan and arabinogalactan-protein epitopes, and in which the cells are trapped in the vicinity of the root tip.






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