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Published on March 4, 2009; 10.1104/pp.109.136531

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Received February 2, 2009
Accepted February 17, 2009

A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II is regulated by B3 domain transcription factors during seed development in Arabidopsis thaliana

Hannetz Roschzttardtz , Ignacia Fuentes , Marcos Vasquez , Claudia Corvalan , Gabriel Leon , Isabel Gomez , Alejandro Araya , Loreto Holuigue , Jesus Vicente-Carbajosa , and Xavier Jordana *

Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile; UMR 5234, CNRS and Universite Victor Segalen Bordeaux2, 33076 Bordeaux Cedex, France; Centro de Biotecnologia y Genomica de Plantas and Departamento de Biotecnologia, ETSI Agronomos, Universidad Politecnica, Ciudad Universitaria, 28040 Madrid, Spain (H.R.; J.V.-C.)

* Corresponding author; email: xjordana{at}bio.puc.cl.

Mitochondrial Complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis thaliana. One of them, SDH2-3, is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here we present data demonstrating that three abscisic acid responsive elements (ABREs) and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABI3, FUS3 and LEC2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the ABREs are shown to be a target for bZIP53, a member of the bZIP family of transcription factors. We show that Group-S1 bZIP53 protein binds the promoter as a heterodimer with group-C bZIP10 or bZIP25. To the best of our knowledge the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.




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The Transcription Factor ABI4 Is a Regulator of Mitochondrial Retrograde Expression of ALTERNATIVE OXIDASE1a
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[Abstract] [Full Text] [PDF]


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