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Published on April 8, 2009; 10.1104/pp.109.136614

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Received February 4, 2009
Accepted April 2, 2009

Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants

Randall W. Shultz *, Tae-Jin Lee , George C. Allen , William F. Thompson , and Linda Hanley-Bowdoin

Department of Molecular and Structural Biochemistry;Department of Horticultural Science; Department of Plant Biology; North Carolina State University, Raleigh, NC, USA

* Corresponding author; email: rwshultz{at}ncsu.edu.

Genome integrity in eukaryotes depends on licensing mechanisms that prevent loading of the minichromosome maintenance complex (MCM2-7) onto replicated DNA during S phase. Although the principle of licensing appears to be conserved across all eukaryotes, the mechanisms that control it vary, and it is not clear how licensing is regulated in plants. In this work, we demonstrated that subunits of the MCM2-7 complex are coordinately expressed during Arabidopsis thaliana development and are abundant in proliferating and endocycling tissues, indicative of a role in DNA replication. We showed that endogenous MCM5 and MCM7 proteins are localized in the nucleus during G1, S and G2 phases of the cell cycle, and are released into the cytoplasmic compartment during mitosis. We also showed that MCM5 and MCM7 are topologically constrained on DNA and that the MCM complex is stable under high-salt conditions. Our results are consistent with a conserved replicative helicase function for the MCM complex in plants, but not with the idea that plants resemble budding yeast by actively exporting the MCM complex from the nucleus to prevent unauthorized origin licensing and re-replication during S phase. Instead, our data show that like other higher eukaryotes, the MCM complex in plants remains in the nucleus throughout most of the cell cycle and is only dispersed in mitotic cells.






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