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Published on April 15, 2009; 10.1104/pp.109.136853

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Received February 9, 2009
Accepted April 8, 2009

A comparative study on the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles

Dror Avisar , Mohamad Abu-Abied , Eduard Belausov , Einat Sadot *, Chris Hawes , and Imogen A. Sparkes

The Institute of Plant Sciences, The Volcani Center, Bet-Dagan 50250, Israel; School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, U.K

* Corresponding author; email: vhesadot{at}agri.gov.il.

Gene families with multiple members are predicted to have individuals with overlapping functions. We examined all the Arabidopsis myosin family members for their involvement in Golgi and other organelles' motility. Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ-tail or tail domains only were fused to fluorescent markers and co-expressed with a Golgi marker in two different plants. We tracked and calculated Golgi body displacement rate in the presence of all myosin truncations and found that tail fragments of myosins MYA1, MYA2, XI-C, XI-E, XI-I and XI-K were the best inhibitors of Golgi body movement in the two plants. Tail fragments of myosins XI-B, XI-F, XI-H, and ATM1 had an inhibitory effect on Golgi bodies only in Nicotiana tabacum while tail fragments of myosin XI-G and ATM2 had a slight effect on Golgi body motility only in Nicotiana benthamiana. The best myosin inhibitors of Golgi body motility were able to arrest mitochondrial movement too. No exclusive co-localization was found between these myosins and Golgi bodies in our system, although the excess of cytosolic signal observed, could mask myosin molecules bound to the surface of the organelle. From the preserved actin filaments found in the presence of eGFP fusions of truncated myosins and the motility of myosin punctae, we conclude that global arrest of acto-myosin derived cytoplasmic streaming had not occurred. Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells.




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