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Published on April 22, 2009; 10.1104/pp.109.138230

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Received March 8, 2009
Accepted April 20, 2009

Reactive oxygen species are involved in brassinosteroids-induced stress tolerance in Cucumis sativus

Xiao-Jian Xia , Yan-Jie Wang , Yan-Hong Zhou , Yuan Tao , Wei-Hua Mao , Kai Shi , Tadao Asami , Zhixiang Chen , and Jing-Quan Yu *

Department of Horticulture, Huajiachi Campus, Zhejiang University, Kaixuan Road 268, Hangzhou, P.R. China 310029; Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Kaixuan Road 268, Hangzhou, 310029 P.R. China; Department of Applied Biological Chemistry, University of Tokyo, Bunkyo Ku, Tokyo 1138657, Japan; Department of Botany & Plant Pathology, Purdue University, West Lafayette, IN 47907-2054, USA

* Corresponding author; email: jqyu{at}zju.edu.cn.

Brassinosteroids (BRs) induce plant tolerance to a wide spectrum of stresses. To study how BR induces stress tolerance, we manipulated the BR levels in cucumber (Cucumis sativus) through a chemical genetics approach and found that BR levels were positively correlated with the tolerance to photo-oxidative and cold stresses and resistance to cucumber mosaic virus (CMV). We also showed that BR treatment enhanced NADPH oxidase activity and elevated H2O2 level in apoplast. H2O2 levels were elevated as early as 3 h and returned to basal levels 3 d after BR treatment. BR-induced H2O2 accumulation was accompanied by increased tolerance to oxidative stress. Inhibition of NADPH oxidase and chemical scavenging of H2O2 reduced BR-induced oxidative and cold tolerance and defense gene expression. BR treatment induced expression of both regulatory genes such as RBOH, MAPK1 and MAPK3, and genes involved in defense and antioxidant responses. These results strongly suggest that elevated H2O2 levels resulting from enhanced NADPH oxidase activity are involved in the BR-induced stress tolerance.






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