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Published on May 15, 2009; 10.1104/pp.109.140905

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Received May 4, 2009
Accepted May 11, 2009

Cis-element- and Transcriptome-based Screening of Root Hair-Specific Genes and their Functional Characterization in Arabidopsis

Su-Kyung Won , Yong-Ju Lee , Ha-Yeon Lee , Yoon-Kyung Heo , Misuk Cho , and Hyung-Taeg Cho *

School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea; Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea

* Corresponding author; email: htcho{at}snu.ac.kr.

Understanding the cellular differentiation of multicellular organisms requires the characterization of genes whose expression is modulated in a cell type–specific manner. The Arabidopsis thaliana root hair cell is one model for studying cellular differentiation. In the present study, root hair cell–specific genes were screened by a series of in silico and experimental filtration procedures. This process included genome-wide screening for genes with a root hair–specific cis-element (RHE) in their promoter; filtering root-specific genes from the RHE-containing genes; further filtering of genes that were suppressed in root hair–defective plant lines; and experimental confirmation by promoter assay. These procedures revealed 19 root hair-specific genes, including many protein kinases and cell wall–related genes, most of which have not been characterized thus far. Functional analyses of these root hair-specific genes with loss-of-function mutants and over-expressing transformants revealed that they play roles in hair growth and morphogenesis. This study demonstrates that a defined cis-element can serve as a filter to screen certain cell type-sepcific genes, and implicates many new root hair–specific genes in root hair development.




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