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First published online November 20, 2009; 10.1104/pp.109.148700

Plant Physiology 152:590-601 (2010)
© 2010 American Society of Plant Biologists

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A Systems Model of Vesicle Trafficking in Arabidopsis Pollen Tubes[W],[OA]

Naohiro Kato*, Hongyu He and Alexander P. Steger

Department of Biological Sciences (N.K., A.P.S.) and Department of Mathematics (H.H.), Louisiana State University, Baton Rouge, Louisiana 70803

A systems model that describes vesicle trafficking during pollen tube growth in Arabidopsis (Arabidopsis thaliana) was constructed. The model is composed of ordinary differential equations that connect the molecular functions of genes expressed in pollen. The current model requires soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs) and small GTPases, Arf or Rab, to reasonably predict tube growth as a function of time. Tube growth depends on vesicle trafficking that transports phospholipid and pectin to the tube tip. The vesicle trafficking genes identified by analyzing publicly available transcriptome data comprised 328 genes. Fourteen of them are up-regulated by the gibberellin signaling pathway during pollen development, which includes the SNARE genes SYP124 and SYP125 and the Rab GTPase gene RABA4D. The model results adequately fit the pollen tube growth of both previously reported wild-type and raba4d knockout lines. Furthermore, the difference of pollen tube growth in syp124/syp125 single and double mutations was quantitatively predicted based on the model analysis. In general, a systems model approach to vesicle trafficking arguably demonstrated the importance of the functional connections in pollen tube growth and can help guide future research directions.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Naohiro Kato (kato{at}lsu.edu).

[W] The online version of this article contains Web-only data.

[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.109.148700

* Corresponding author; e-mail kato{at}lsu.edu.

Received October 2, 2009; accepted November 16, 2009; published November 20, 2009.

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