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DEVELOPMENT AND HORMONE ACTION

Peptide and Amino Acid Transporters Are Differentially Regulated during Seed Development and Germination in Faba Bean¹

Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, D-06466 Germany (M.M., L.B., A.T., H.W., U.W.); and Institut für Pflanzenwissenschaften, Universität Bern, Bern, CH-3008 Switzerland (D.D., D.R.)

Two peptide transporter (PTR) homologs have been isolated from developing seeds of faba bean (Vicia faba). VfPTR1 was shown to be a functional peptide transporter through complementation of a yeast mutant. Expression patterns of VfPTR1 and VfPTR2 as well as of the amino acid permease VfAAP1 (Miranda et al., 2001) were compared throughout seed development and germination. In developing seeds, the highest levels of VfPTR1 transcripts were reached during midcotyledon development, whereas VfAAP1 transcripts were most abundant during early cotyledon development, before the appearance of storage protein gene transcripts, and were detectable until late cotyledon development. During early germination, VfPTR1 mRNA appeared first in cotyledons and later, during seedling growth, also in axes and roots. Expression of VfPTR2 and VfAAP1 was delayed compared with VfPTR1, and was restricted to the nascent organs of the seedlings. Localization of VfPTR1 transcripts showed that this PTR is temporally and spatially regulated during cotyledon development. In germinating seeds, VfPTR1 mRNA was localized in root hairs and root epidermal cells, suggesting a role in nutrient uptake from the soil. In seedling roots, VfPTR1 was repressed by a dipeptide and by an amino acid, whereas nitrate was without influence.

¹ This work was supported by the Deutsche Forschungsgemein-schaft (project no. WE1614/5–1)

² Present address: Biology Department, University of Victoria, P.O. Box 3020, STN CSC, Victoria, V8W 3N5, British Columbia, Canada

³ In the present report, AtPTR2-A (Steiner et al., 1994) will not be regarded as a plant PTR following the author's correction (Steiner et al., 2000). The denomination AtPTR2 will be used as a synonym for AtPTR2-B (Song et al., 1996), AtNTR1 (Frommer et al., 1994a), and AtOPT1 as in Rentsch et al. (1998), as they all correspond to the same PTR isoform.

^* Corresponding author; e-mail miranda{at}uvic.ca; fax 250–7216611.

Received March 28, 2003; returned for revision April 25, 2003; accepted May 6, 2003.

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