First published online October 2, 2003; 10.1104/pp.103.025122
Plant Physiology 133:1102-1110 (2003)
© 2003 American Society of Plant Biologists
GENETICS, GENOMICS, AND MOLECULAR EVOLUTION
Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control1
Erin L. Connolly*,
Nathan H. Campbell,
Natasha Grotz,
Charis L. Prichard2 and
Mary Lou Guerinot
Department of Biological Sciences, Coker Life Sciences, University of South Carolina, Columbia, South Carolina 29208 (E.L.C., N.H.C., C.L.P.); and Department of Biological Sciences, 6044 Gilman, Dartmouth College, Hanover, New Hampshire 03755 (N.G., M.L.G.)
The Arabidopsis FRO2 gene encodes the low-iron-inducible ferric chelate reductase responsible for reduction of iron at the root surface. Here, we report that FRO2 and IRT1, the major transporter responsible for high-affinity iron uptake from the soil, are coordinately regulated at both the transcriptional and posttranscriptional levels. FRO2 and IRT1 are induced together following the imposition of iron starvation and are coordinately repressed following iron resupply. Steady-state mRNA levels of FRO2 and IRT1 are also coordinately regulated by zinc and cadmium. Like IRT1, FRO2 mRNA is detected in the epidermal cells of roots, consistent with its proposed role in iron uptake from the soil. FRO2 mRNA is detected at high levels in the roots and shoots of 35S-FRO2 transgenic plants. However, ferric chelate reductase activity is only elevated in the 35S-FRO2 plants under conditions of iron deficiency, indicating that FRO2 is subject to posttranscriptional regulation, as shown previously for IRT1. Finally, the 35S-FRO2 plants grow better on low iron as compared with wild-type plants, supporting the idea that reduction of ferric iron to ferrous iron is the rate-limiting step in iron uptake.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.025122.
1 This work was supported by the National Science Foundation (grant no. IBN–9974837 to M.L.G.) and by the U.S. Department of Agriculture National Research Initiatives Competitive Grants Program (grant nos. 9900598 and 0188925 to E.L.C.).
2 Present address: University of South Carolina, School of Medicine, Columbia, SC 29208.
* Corresponding author; e-mail erinc{at}biol.sc.edu; fax 803–777–4002.
Received April 8, 2003;
returned for revision June 17, 2003;
accepted July 2, 2003.
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