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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Overexpression of SOD2 Increases Salt Tolerance of Arabidopsis¹

Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China

The yeast (Schizosaccharomyces pombe) SOD2 (Sodium2) gene was introduced into Arabidopsis under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on medium containing kanamycin. Southern- and northern-blot analyses confirmed that SOD2 was transferred into the Arabidopsis genome. There were no obvious morphological or developmental differences between the transgenic and wild-type (wt) plants. Several transgenic homozygous lines and wt plants (control) were evaluated for salt tolerance and gene expression. Overexpression of SOD2 in Arabidopsis improved seed germination and seedling salt tolerance. Analysis of Na⁺ and K⁺ contents of the symplast and apoplast in the parenchyma cells of the root cortex and mesophyll cells in the spongy tissue of the leaf showed that transgenic lines accumulated less Na⁺ and more K⁺ in the symplast than the wt plants did. The photosynthetic rate and the fresh weight of the transgenic lines were distinctly higher than that of wt plants after NaCl treatment. Results from different tests indicated that the expression of the SOD2 gene promoted a higher level of salt tolerance in vivo in transgenic Arabidopsis plants.

² Present address: Shanghai Institute of Plant Physiology and Ecology, The Chinese Academy of Science, 300 Fenglin Road, Shanghai 200032, China.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.026062.

^* Corresponding author; e-mail zhangh{at}sdnu.edu.cn; fax 86-531-6180764.

Received April 28, 2003; returned for revision June 11, 2003; accepted September 11, 2003.

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