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DEVELOPMENT AND HORMONE ACTION

The Arabidopsis Locus RCB Mediates Upstream Regulation of Mitotic Gene Expression¹

Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Gent, Belgium (K.H., T.B., L.C., D.I.); CropDesign N.V., B-9052 Gent, Belgium (C.R.); Université de Liège, Sart-Tilman, B-4000 Liège, Belgium (S.M.); and Institut National de la Recherche Agronomique, Centre de Versailles, Route de Saint-Cyr, F-78026, France (O.G.)

Transcriptional regulation of cell cycle regulatory genes, such as B-type cyclins, is tightly linked with the mitotic activity in the meristems. To study the regulation of a B-type cyclin gene, a targeted genetic approach was undertaken. An Arabidopsis line containing a fusion construct between the CYCB1;1 promoter and a bacterial -glucuronidase marker gene (uidA) was used in ethyl methanesulfonate mutagenesis. The mutants were screened for altered CYCB1;1::uidA expression patterns. In a reduced CYCB1;1 expression mutant (rcb), the CYCB1;1::uidA expression was severely affected, being excluded from the shoot and root apical meristems and leaf primordia and shifted to cells associated with root cap and stomata. In addition to the overall reduction of the endogenous CYCB1;1 transcript levels, other G2-to-M phase-specific genes were also down-regulated by the mutation. In the mutant plants, the inflorescence stem growth was reduced, indicating low meristem activity. Based on the altered CYCB1;1::uidA expression patterns in rcb root meristem, a model is proposed for RCB that mediates the tissue specificity of CYCB1;1 promoter activity.

² Present address: Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021.

³ Present address: University of East Anglia, Norwich NR4 7TJ, UK.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.027128.

^* Corresponding author; e-mail dirk.inze{at}psb.ugent.be; fax 32-9-3313809.

Received May 21, 2003; returned for revision June 25, 2003; accepted September 9, 2003.

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