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First published online November 20, 2003; 10.1104/pp.103.029363

Plant Physiology 133:1968-1978 (2003)
© 2003 American Society of Plant Biologists

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BIOENERGETICS AND PHOTOSYNTHESIS

Identification of AtNDI1, an Internal Non-Phosphorylating NAD(P)H Dehydrogenase in Arabidopsis Mitochondria1

Catherine S. Moore, Rebecca J. Cook-Johnson, Charlotta Rudhe, James Whelan, David A. Day, Joseph T. Wiskich and Kathleen L. Soole*

School of Biological Sciences, Flinders University of South Australia, G.P.O. Box 2100, Adelaide, South Australia 5001, Australia (C.S.M., R.J.C.-J., J.T.W., K.L.S.); Department of Biochemistry and Biophysics, Stockholm University, Stockholm S106 91, Sweden (C.R.); and Plant Molecular Biology Group, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia (J.W., D.A.D.)

Plant mitochondria contain non-phosphorylating NAD(P)H dehydrogenases (DHs) that are not found in animal mitochondria. The physiological function, substrate specificity, and location of enzymes within this family have yet to be conclusively determined. We have linked genome sequence information to protein and biochemical data to identify that At1g07180 (SwissProt Q8GWA1) from the Arabidopsis Genome Initiative database encodes AtNDI1, an internal NAD(P)H DH in Arabidopsis mitochondria. Three lines of evidence are presented: (a) The predicted protein sequence of AtNDI1 has high homology with other designated NAD(P)H DHs from microorganisms, (b) the capacity for matrix NAD(P)H oxidation via the rotenone-insensitive pathway is significantly reduced in the Atndi1 mutant plant line, and (c) the in vitro translation product of AtNDI1 is imported into isolated mitochondria and located on the inside of the inner membrane.

1 This work was fully funded by the Australian Research Council. Experiments were performed at Flinders University, with the exception of the in vitro translation and protein import studies, which were conducted at the University of Western Australia, Perth.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.029363.

* Corresponding author; e-mail kathleen.soole{at}flinders.edu.au; fax 61-0-8-8201-3015.

Received June 30, 2003; returned for revision July 18, 2003; accepted August 17, 2003.




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