First published online March 26, 2004; 10.1104/pp.103.031971
Plant Physiology 134:1574-1585 (2004)
© 2004 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
Regulation of Flower Development in Arabidopsis by SCF Complexes1
Weimin Ni,
Daoxin Xie,
Lawrence Hobbie,
Baomin Feng,
Dazhong Zhao,
Joseph Akkara and
Hong Ma*
Department of Biology and the Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802 (W.N., B.F., D.Z., J.A., H.M.); Institute of Molecular and Cell Biology, Singapore 11609 (D.X.); and Department of Biology, Adelphi University, Garden City, New York 11530 (L.H.)
SCF complexes are the largest and best studied family of E3 ubiquitin protein ligases that facilitate the ubiquitylation of proteins targeted for degradation. The SCF core components Skp1, Cul1, and Rbx1 serve in multiple SCF complexes involving different substrate-specific F-box proteins that are involved in diverse processes including cell cycle and development. In Arabidopsis, mutations in the F-box gene UNUSUAL FLORAL ORGANS (UFO) result in a number of defects in flower development. However, functions of the core components Cul1 and Rbx1 in flower development are poorly understood. In this study we analyzed floral phenotypes caused by altering function of Cul1 or Rbx1, as well as the effects of mutations in ASK1 and ASK2. Plants homozygous for a point mutation in the AtCUL1 gene showed reduced floral organ number and several defects in each of the four whorls. Similarly, plants with reduced AtRbx1 expression due to RNA interference also exhibited floral morphological defects. In addition, compared to the ask1 mutant, plants homozygous for ask1 and heterozygous for ask2 displayed enhanced reduction of B function, as well as other novel defects of flower development, including carpelloid sepals and an inhibition of petal development. Genetic analyses demonstrate that AGAMOUS (AG) is required for the novel phenotypes observed in the first and second whorls. Furthermore, the genetic interaction between UFO and AtCUL1 supports the idea that UFO regulates multiple aspects of flower development as a part of SCF complexes. These results suggest that SCF complexes regulate several aspects of floral development in Arabidopsis.
1 This work was supported by the National Science Foundation (grant nos. MCB–9896340 and MCB–0092075 to H.M. and IBN–998926 to L.H.), and by funds from the Department of Biology and the Huck Institutes of Life Sciences at the Pennsylvania State University.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.031971.
* Corresponding author; e-mail hxm16{at}psu.edu; fax 814–863–1357.
Received August 19, 2003;
returned for revision November 3, 2003;
accepted January 12, 2004.
This article has been cited by other articles:
|
|
|
|
 
C. L.H. Hord, Y.-J. Sun, L. J. Pillitteri, K. U. Torii, H. Wang, S. Zhang, and H. Ma
Regulation of Arabidopsis Early Anther Development by the Mitogen-Activated Protein Kinases, MPK3 and MPK6, and the ERECTA and Related Receptor-Like Kinases
Mol Plant,
July 1, 2008;
1(4):
645 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Chae, Q. K.-G. Tan, T. A. Hill, and V. F. Irish
An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development
Development,
April 1, 2008;
135(7):
1235 - 1245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. H. Gonzalez-Carranza, U. Rompa, J. L. Peters, A. M. Bhatt, C. Wagstaff, A. D. Stead, and J. A. Roberts
HAWAIIAN SKIRT: An F-Box Gene That Regulates Organ Fusion and Growth in Arabidopsis
Plant Physiology,
July 1, 2007;
144(3):
1370 - 1382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Moon, Y. Zhao, X. Dai, W. Zhang, W. M. Gray, E. Huq, and M. Estelle
A New CULLIN 1 Mutant Has Altered Responses to Hormones and Light in Arabidopsis
Plant Physiology,
February 1, 2007;
143(2):
684 - 696.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yang, L. Timofejeva, H. Ma, and C. A. Makaroff
The Arabidopsis SKP1 homolog ASK1 controls meiotic chromosome remodeling and release of chromatin from the nuclear membrane and nucleolus
J. Cell Sci.,
September 15, 2006;
119(18):
3754 - 3763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, Y. Sun, L. Timofejeva, C. Chen, U. Grossniklaus, and H. Ma
Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM1 (DYT1) encoding a putative bHLH transcription factor
Development,
August 15, 2006;
133(16):
3085 - 3095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Moon, G. Parry, and M. Estelle
The Ubiquitin-Proteasome Pathway and Plant Development
PLANT CELL,
December 1, 2004;
16(12):
3181 - 3195.
[Full Text]
[PDF]
|
|
|
|
|
